BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 27145

Title: Backbone 1H, 13C, 15N assignments for the chromoshadow domain of S. pombe Swi6   PubMed: 28942089

Authors: Gross, John; Sanulli, Serena; Tibble, Ryan; Isaac, R Stefan; Narlikar, Geeta

Citation: Stefan Isaac, R.; Sanulli, Serena; Tibble, Ryan; Hornsby, Michael; Ravalin, Matthew; Craik, Charles; Gross, John; Narlikar, Geeta. "Biochemical basis for distinct roles of the heterochromatin proteins Swi6 and Chp2"  J. Mol. Biol. ., 30449-30449 (2017).

Assembly members:
Swi6, polymer, 69 residues, Formula weight is not available

Natural source:   Common Name: fission yeast   Taxonomy ID: 4896   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Schizosaccharomyces pombe

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Swi6: GKQVENYDSWEDLVSSIDTI ERKDDGTLEIYLTWKNGAIS HHPSTITNKKCPQKMLQFYE SHLTFRENE

Data sets:
Data typeCount
13C chemical shifts106
15N chemical shifts55
1H chemical shifts55

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1chromatin-associated portein swi61

Entities:

Entity 1, chromatin-associated portein swi6 69 residues - Formula weight is not available

1   GLYLYSGLNVALGLUASNTYRASPSERTRP
2   GLUASPLEUVALSERSERILEASPTHRILE
3   GLUARGLYSASPASPGLYTHRLEUGLUILE
4   TYRLEUTHRTRPLYSASNGLYALAILESER
5   HISHISPROSERTHRILETHRASNLYSLYS
6   CYSPROGLNLYSMETLEUGLNPHETYRGLU
7   SERHISLEUTHRPHEARGGLUASNGLU

Samples:

Swi6_CSD: Swi6, [U-100% 13C; U-100% 15N], 250 uM; HEPES pH 7.8 20 mM; potassium chloride 150 mM; DTT 2 mM

sample_conditions_1: ionic strength: 150 mM; pH: 7.5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCSwi6_CSDisotropicsample_conditions_1
3D CBCA(CO)NHSwi6_CSDisotropicsample_conditions_1
3D HNCASwi6_CSDisotropicsample_conditions_1

Software:

SPARKY v3.131, Bahrami, Markley, Assadi, and Eghbalnia, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Goddard - chemical shift assignment, peak picking, processing

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker DRX 500 MHz

Related Database Links:

UNP P40381