BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 27152

Title: 3Jcgn & 3Jcgc' scalar couplings for CUG-BP2 RRM3 H429A mutant in complex with (5'-R(*UP*UP*UP*AP*A)-3')   PubMed: 28935965

Authors: Diarra dit Konte, N.; Damberger, F.; Allain, F.

Citation: Diarra Dit Konte, Nana; Krepl, Miroslav; Damberger, Fred; Ripin, Nina; Duss, Olivier; Sponer, Jiri; Allain, Frederic H-T. "Aromatic side-chain conformational switch on the surface of the RNA Recognition Motif enables RNA discrimination"  Nat. Commun. 8, 654-654 (2017).

Assembly members:
entity_1, polymer, 114 residues, Formula weight is not available
entity_2, polymer, 5 residues, 1531.952 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity_1: MGSSHHHHHHSSGLVPRGSH MQKEGPEGANLFIYALPQEF GDQDILQMFMPFGNVISAKV FIDKQTNLSKCFGFVSYDNP VSAQAAIQAMNGFQIGMKRL KVQLKRSKNDSKPW
entity_2: UUUAA

Data sets:
Data typeCount
coupling constants19

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_22

Entities:

Entity 1, entity_1 114 residues - Formula weight is not available

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METGLNLYSGLUGLYPROGLUGLYALAASN
4   LEUPHEILETYRALALEUPROGLNGLUPHE
5   GLYASPGLNASPILELEUGLNMETPHEMET
6   PROPHEGLYASNVALILESERALALYSVAL
7   PHEILEASPLYSGLNTHRASNLEUSERLYS
8   CYSPHEGLYPHEVALSERTYRASPASNPRO
9   VALSERALAGLNALAALAILEGLNALAMET
10   ASNGLYPHEGLNILEGLYMETLYSARGLEU
11   LYSVALGLNLEULYSARGSERLYSASNASP
12   SERLYSPROTRP

Entity 2, entity_2 5 residues - 1531.952 Da.

1   UUUAA

Samples:

sample_1: 5'-UUUAA-3' RNA, non-labeled, 1.95 mM; CUG-BP2 third RNA Recognition Motif, [U-99% 13C; U-99% 15N], 0.65 mM; H2O 95 % v/v; D2O 5 % v/v; potassium phosphate 20 mM; sodium chloride 10 mM; beta-mercaptoethanol 10 mM; DEPC 0.1 % v/v

sample_conditions_1: ionic strength: 130 mM; pH: 5.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
spin-echo difference constant time 15N-HSQCsample_1isotropicsample_conditions_1
spin-echo difference constant time HN(CO)CGsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment, data analysis

TOPSPIN, Bruker Biospin - collection, processing

NMR spectrometers:

  • Bruker AvanceIII 600 MHz
  • Bruker AvanceIII HD 600 MHz

Related Database Links: