BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 27162

Title: NMR resonance assignments of the EVH1-domain of Neurofibromin's recruitment factor Spred1   PubMed: 28831766

Authors: Fuehrer, Sebastian; Ahammer, Linda; Ausserbichler, Angela; Scheffzek, Klaus; Dunzendorfer-Matt, Theresia; Tollinger, Martin

Citation: Fuehrer, Sebastian; Ahammer, Linda; Ausserbichler, Angela; Scheffzek, Klaus; Dunzendorfer-Matt, Theresia; Tollinger, Martin. "NMR resonance assignments of the EVH1 domain of neurofibromin's recruitment factor Spred1"  Biomol. NMR Assignments ., .-. (2017).

Assembly members:
Spred1-EVH1, polymer, 120 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Spred1-EVH1: MGSYARVRAVVMTRDDSSGG WLPLGGSGLSSVTVFKVPHQ EENGCADFFIRGERLRDKMV VLECMLKKDLIYNKVTPTFH HWKIDDKKFGLTFQSPADAR AFDRGIRRAIEDISQGCPES

Data sets:
Data typeCount
13C chemical shifts461
15N chemical shifts117
1H chemical shifts723

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Spred1-EVH11

Entities:

Entity 1, Spred1-EVH1 120 residues - Formula weight is not available

The first two residues, Met-Gly, are derived from the plasmid

1   METGLYSERTYRALAARGVALARGALAVAL
2   VALMETTHRARGASPASPSERSERGLYGLY
3   TRPLEUPROLEUGLYGLYSERGLYLEUSER
4   SERVALTHRVALPHELYSVALPROHISGLN
5   GLUGLUASNGLYCYSALAASPPHEPHEILE
6   ARGGLYGLUARGLEUARGASPLYSMETVAL
7   VALLEUGLUCYSMETLEULYSLYSASPLEU
8   ILETYRASNLYSVALTHRPROTHRPHEHIS
9   HISTRPLYSILEASPASPLYSLYSPHEGLY
10   LEUTHRPHEGLNSERPROALAASPALAARG
11   ALAPHEASPARGGLYILEARGARGALAILE
12   GLUASPILESERGLNGLYCYSPROGLUSER

Samples:

sample_1: Spred1-EVH1, [U-99% 13C; U-99% 15N], 0.6-0.8 mM; sodium phosphate 20 mM; sodium chloride 50 mM

sample_conditions_1: ionic strength: 70 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D (H)CC(CO)NH-TOCSYsample_1isotropicsample_conditions_1
3D H(CC)(CO)NH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Agilent DD2 500 MHz

Related Database Links:

NCBI NP_689807.1