BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 27185

Title: ProXp-ala free form

Authors: Danhart, Eric

Citation: Danhart, Eric; Bakhtina, Marina; Cantara, William; Kuzmishin, Alexandra; Ma, Xiao; Sanford, Brianne; Kosutic, Marija; Goto, Yuki; Suga, Hiroaki; Nakanishi, Kotrao; Micura, Ronald; Foster, Mark; Musier-Forsyth, Karin. "Conformational and chemical selection by a trans-acting editing domain"  Proc. Natl. Acad. Sci. U.S.A. ., .-..

Assembly members:
ProXp-ala, polymer, 171 residues, Formula weight is not available

Natural source:   Common Name: Caulobacter vibrioides   Taxonomy ID: 155892   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Caulobacter crescentus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
ProXp-ala: GSHMKTRADLFAFFDAHGVD HKTLDHPPVFRVEEGLEIKA AMPGGHTKNLFLKDAKGQLW LISALGETTIDLKKLHHVIG SGRLSFGPQEMMLETLGVTP GSVTAFGLINDTEKRVRFVL DKALADSDPVNFHPLKNDAT TAVSQAGLRRFLAALGVEPM IVDFAAMEVVG

Data sets:
Data typeCount
13C chemical shifts182
15N chemical shifts156
1H chemical shifts156

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ProXp-ala1

Entities:

Entity 1, ProXp-ala 171 residues - Formula weight is not available

1   GLYSERHISMETLYSTHRARGALAASPLEU
2   PHEALAPHEPHEASPALAHISGLYVALASP
3   HISLYSTHRLEUASPHISPROPROVALPHE
4   ARGVALGLUGLUGLYLEUGLUILELYSALA
5   ALAMETPROGLYGLYHISTHRLYSASNLEU
6   PHELEULYSASPALALYSGLYGLNLEUTRP
7   LEUILESERALALEUGLYGLUTHRTHRILE
8   ASPLEULYSLYSLEUHISHISVALILEGLY
9   SERGLYARGLEUSERPHEGLYPROGLNGLU
10   METMETLEUGLUTHRLEUGLYVALTHRPRO
11   GLYSERVALTHRALAPHEGLYLEUILEASN
12   ASPTHRGLULYSARGVALARGPHEVALLEU
13   ASPLYSALALEUALAASPSERASPPROVAL
14   ASNPHEHISPROLEULYSASNASPALATHR
15   THRALAVALSERGLNALAGLYLEUARGARG
16   PHELEUALAALALEUGLYVALGLUPROMET
17   ILEVALASPPHEALAALAMETGLUVALVAL
18   GLY

Samples:

sample_1: ProXp-ala, [U-100% 13C; U-100% 15N], 0.7 mM

sample_conditions_1: ionic strength: 0.16 M; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TOPSPIN, Bruker Biospin - collection

NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker DRX 600 MHz

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