BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 27193

Title: Backbone 1H, 15N chemical shift for Y39E EVH1

Authors: Acevedo, Lucila; Greenwood, Alexander; Nicholson, Linda

Citation: Acevedo, Lucila; Greenwood, Alexander; Nicholson, Linda. "A Noncanonical Binding Site in the EVH1 Domain of VASP Regulates Its Interactions with the Proline Rich Region of Zyxin"  Biochemistry ., .-..

Assembly members:
Y39E_EVH1, polymer, 121 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Y39E_EVH1: GPGGRMSSETVICSSRATVM LYDDGNKRWLPAGTGPQAFS RVQIEHNPTANSFRVVGRKM QPDQQVVINCAIVRGVKYNQ ATPNFHQWRDARQVWGLNFG SKEDAAQFAAGMASALEALE G

Data sets:
Data typeCount
15N chemical shifts123
1H chemical shifts134

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Y39E EVH11

Entities:

Entity 1, Y39E EVH1 121 residues - Formula weight is not available

GPGGRMS is an artifact of cloning and 3cpro recognition, Y39E EVH1 is a mutation of Y39, and this contains the sequence from 2-115.

1   GLYPROGLYGLYARGMETSERSERGLUTHR
2   VALILECYSSERSERARGALATHRVALMET
3   LEUTYRASPASPGLYASNLYSARGTRPLEU
4   PROALAGLYTHRGLYPROGLNALAPHESER
5   ARGVALGLNILEGLUHISASNPROTHRALA
6   ASNSERPHEARGVALVALGLYARGLYSMET
7   GLNPROASPGLNGLNVALVALILEASNCYS
8   ALAILEVALARGGLYVALLYSTYRASNGLN
9   ALATHRPROASNPHEHISGLNTRPARGASP
10   ALAARGGLNVALTRPGLYLEUASNPHEGLY
11   SERLYSGLUASPALAALAGLNPHEALAALA
12   GLYMETALASERALALEUGLUALALEUGLU
13   GLY

Samples:

sample_1: Y39E EVH1, [U-99% 15N], 0.23 mM; potassium chloride 50 mM; potassium phosphate 20 mM; TCEP 1 mM; sodium azide 5 mM

sample_conditions_1: ionic strength: 94.76 mM; pH: 6.7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

VNMRJ, Varian - collection

SPARKY, Goddard - data analysis

NMR spectrometers:

  • Varian INOVA 600 MHz

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