BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 27219

Title: ssNMR assignment of membrane embedded NaK channel (ion-free conformer)   PubMed: 29459730

Authors: Shi, Chaowei; He, Yao; Hendriks, Kitty; de Groot, Bert L.; Tian, Changlin; Lange, Adam; Sun, Han

Citation: Shi, Chaowei; He, Yao; Hendriks, Kitty; de Groot, Bert; Cai, Xiaoying; Tian, Changlin; Lange, Adam; Sun, Han. "A single NaK channel conformation is not enough for non-selective ion conduction"  Nat. Commun. 9, 717-717 (2018).

Assembly members:
NaK_channel, polymer, 106 residues, Formula weight is not available

Natural source:   Common Name: Bacillus cereus   Taxonomy ID: 1396   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus cereus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
NaK_channel: MGHHHHHHMAWKDKEFQVLF VLTILTLISGTIFYSTVEGL RPIDALYFSVVTLTTVGDGN FSPQTDFGKIFTILYIFIGI GLVFGFIHKLAVNVQLPSIL SNRKKE

Data sets:
Data typeCount
13C chemical shifts231
15N chemical shifts55

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NaK channel1

Entities:

Entity 1, NaK channel 106 residues - Formula weight is not available

1   METGLYHISHISHISHISHISHISMETALA
2   TRPLYSASPLYSGLUPHEGLNVALLEUPHE
3   VALLEUTHRILELEUTHRLEUILESERGLY
4   THRILEPHETYRSERTHRVALGLUGLYLEU
5   ARGPROILEASPALALEUTYRPHESERVAL
6   VALTHRLEUTHRTHRVALGLYASPGLYASN
7   PHESERPROGLNTHRASPPHEGLYLYSILE
8   PHETHRILELEUTYRILEPHEILEGLYILE
9   GLYLEUVALPHEGLYPHEILEHISLYSLEU
10   ALAVALASNVALGLNLEUPROSERILELEU
11   SERASNARGLYSLYSGLU

Samples:

sample_1: NaK channel, [U-99% 13C; U-99% 15N], 10 mg; TRIS 20 mM; sodium chloride 50 mM; Asolectin 5 mg

sample_conditions_1: ionic strength: 50 mM; pH: 8; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D PDSDsample_1solidsample_conditions_1
2D NCAsample_1solidsample_conditions_1
2D NCOsample_1solidsample_conditions_1
3D CANCOsample_1solidsample_conditions_1
3D NCOCAsample_1solidsample_conditions_1
3D NCOCACBsample_1solidsample_conditions_1
2D CANCOCAsample_1solidsample_conditions_1
3D NCACXsample_1solidsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

SPARKY, Goddard - data analysis

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

UniProt Q81HW2