BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 27236

Title: Resonance assignment of the central conserved region (domains 8 to 14) of human tropoelastin

Authors: Reichheld, Sean; Sharpe, Simon

Citation: Reichheld, Sean; Sharpe, Simon. "Resonance assignment and spectral density mapping of the central conserved region of tropoelastin"  J. Biomol. NMR ., .-..

Assembly members:
hTE-CCR, polymer, 123 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
hTE-CCR: GAVVPQPGAGVKPGKVPGVG LPGVYPGGVLPGARFPGVGV LPGVPTGAGVKPKAPGVGGA FAGIPGVGPFGGPQPGVPLG YPIKAPKLPGGYGLPYTTGK LPYGYGPGGVAGAAGKAGYP TGT

Data sets:
Data typeCount
13C chemical shifts324
15N chemical shifts118
1H chemical shifts374

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hTE-CCR monomer1

Entities:

Entity 1, hTE-CCR monomer 123 residues - Formula weight is not available

1   GLYALAVALVALPROGLNPROGLYALAGLY
2   VALLYSPROGLYLYSVALPROGLYVALGLY
3   LEUPROGLYVALTYRPROGLYGLYVALLEU
4   PROGLYALAARGPHEPROGLYVALGLYVAL
5   LEUPROGLYVALPROTHRGLYALAGLYVAL
6   LYSPROLYSALAPROGLYVALGLYGLYALA
7   PHEALAGLYILEPROGLYVALGLYPROPHE
8   GLYGLYPROGLNPROGLYVALPROLEUGLY
9   TYRPROILELYSALAPROLYSLEUPROGLY
10   GLYTYRGLYLEUPROTYRTHRTHRGLYLYS
11   LEUPROTYRGLYTYRGLYPROGLYGLYVAL
12   ALAGLYALAALAGLYLYSALAGLYTYRPRO
13   THRGLYTHR

Samples:

sample_1: hTE-CCR, [U-13C; U-15N], 3.5 mM; potassium phosphate 50 mM; sodium chloride 350 mM; D2O 10%; H2O 90%

sample_conditions_1: ionic strength: 0.35 M; pH: 7; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D NOESY-HSQCsample_1isotropicsample_conditions_1
3D HSQC-TOCSYsample_1isotropicsample_conditions_1
3D CBCANHsample_1isotropicsample_conditions_1
3D HNNsample_1isotropicsample_conditions_1
3D HN(CO)Nsample_1isotropicsample_conditions_1
3D HCANsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CcpNMR, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

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