BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 27243

Title: RPT1 region of INI1/SNF5/SMARCB1_HUMAN - SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1

Authors: Girvin, Mark; Cahill, Sean; Harris, RIchard; Cowburn, David; Spira, Menachem; Wu, Xuhong; Prakash, Rajesh; Brenowitz, Michael; Almo, Steven; Kalpana, Ganjam

Citation: Girvin, Mark; Cahill, Sean; Harris, RIchard; Cowburn, David; Spira, Menachem; Wu, Xuhong; Prakash, Rajesh; Brenowitz, Michael; Almo, Steven; Kalpana, Ganjam. "RPT1 region of INI1/SNF5/SMARCB1_HUMAN - SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1"  Not known ., .-..

Assembly members:
Rpt1-ini1, polymer, 83 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Rpt1-ini1: PEVLVPIRLDMEIDGQKLRD AFTWNMNEKLMTPEMFSEIL CDDLDLNPLTFVPAIASAIR QQIESYPTDSILEDQSDQRV IIK

Data sets:
Data typeCount
13C chemical shifts387
15N chemical shifts86
1H chemical shifts627

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Rpt1/ini11

Entities:

Entity 1, Rpt1/ini1 83 residues - Formula weight is not available

1   PROGLUVALLEUVALPROILEARGLEUASP
2   METGLUILEASPGLYGLNLYSLEUARGASP
3   ALAPHETHRTRPASNMETASNGLULYSLEU
4   METTHRPROGLUMETPHESERGLUILELEU
5   CYSASPASPLEUASPLEUASNPROLEUTHR
6   PHEVALPROALAILEALASERALAILEARG
7   GLNGLNILEGLUSERTYRPROTHRASPSER
8   ILELEUGLUASPGLNSERASPGLNARGVAL
9   ILEILELYS

Samples:

sample_1: Rpt1/ini1, [U-95% 13C; U-95% 15N], 250 uM; sodium phosphate 10 mM; sodium chloride 150 mM; EDTA 1 mM; TCEP 5 mM; TSP 0.01 ± 0.01 mM

sample_conditions_1: ionic strength: 0.16 M; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1

Software:

ARIA, Linge, O'Donoghue and Nilges - data analysis

NMR spectrometers:

  • Agilent INOVA 600 MHz
  • Bruker Avance 900 MHz

Related Database Links:

Uniprot Q12824