BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 27252

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for the yeast ADP/ATP carrier 3

Authors: Bruschweiler, Sven; Chou, James

Citation: Bruschweiler, Sven; Yang, Qin; Run, Changqing; Chou, James. "Substrate Modulated Dynamics of the ADP/ATP Transporter Revealed by NMR Relaxation Dispersion"  Nat. Struct. Biol. ., .-..

Assembly members:
yAAC3, polymer, 307 residues, Formula weight is not available

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
yAAC3: RGSDAKQQETNFAINFLMGG VSAAIAKTAASPIERVKILI QNQDEMIKQGTLDKKYSGIV DAFKRTAKQEGLISFWRGNT ANVIRYFPTQALNFAFKDKI KLMFGFKKEEGYGKWFAGNL ASGGAAGALSLLFVYSLDFA RTRLAADAKSSKKGGARQFN GLTDVYKKTLKSDGIAGLYR GFMPSVVGIVVYRGLYFGMF DSLKPLVLTGSLDGSFLASF LLGWVVTTGASTVSYPLDTV RRRMMMTSGQAVKYNGAIDA LKKIVASEGVGSLFKGAGAN ILRSVAGAGVISMYDQLQMI LFGKKFK

Data sets:
  • assigned_chemical_shifts
Data typeCount
molecule interaction chemical shift values614

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1yAAC31

Entities:

Entity 1, yAAC3 307 residues - Formula weight is not available

1   ARGGLYSERASPALALYSGLNGLNGLUTHR
2   ASNPHEALAILEASNPHELEUMETGLYGLY
3   VALSERALAALAILEALALYSTHRALAALA
4   SERPROILEGLUARGVALLYSILELEUILE
5   GLNASNGLNASPGLUMETILELYSGLNGLY
6   THRLEUASPLYSLYSTYRSERGLYILEVAL
7   ASPALAPHELYSARGTHRALALYSGLNGLU
8   GLYLEUILESERPHETRPARGGLYASNTHR
9   ALAASNVALILEARGTYRPHEPROTHRGLN
10   ALALEUASNPHEALAPHELYSASPLYSILE
11   LYSLEUMETPHEGLYPHELYSLYSGLUGLU
12   GLYTYRGLYLYSTRPPHEALAGLYASNLEU
13   ALASERGLYGLYALAALAGLYALALEUSER
14   LEULEUPHEVALTYRSERLEUASPPHEALA
15   ARGTHRARGLEUALAALAASPALALYSSER
16   SERLYSLYSGLYGLYALAARGGLNPHEASN
17   GLYLEUTHRASPVALTYRLYSLYSTHRLEU
18   LYSSERASPGLYILEALAGLYLEUTYRARG
19   GLYPHEMETPROSERVALVALGLYILEVAL
20   VALTYRARGGLYLEUTYRPHEGLYMETPHE
21   ASPSERLEULYSPROLEUVALLEUTHRGLY
22   SERLEUASPGLYSERPHELEUALASERPHE
23   LEULEUGLYTRPVALVALTHRTHRGLYALA
24   SERTHRVALSERTYRPROLEUASPTHRVAL
25   ARGARGARGMETMETMETTHRSERGLYGLN
26   ALAVALLYSTYRASNGLYALAILEASPALA
27   LEULYSLYSILEVALALASERGLUGLYVAL
28   GLYSERLEUPHELYSGLYALAGLYALAASN
29   ILELEUARGSERVALALAGLYALAGLYVAL
30   ILESERMETTYRASPGLNLEUGLNMETILE
31   LEUPHEGLYLYSLYSPHELYS

Samples:

sample_1: yAAC3, [U-100% 13C; U-100% 15N], 0.8 mM

sample_2: yAAC3, [U-100% 13C; U-100% 15N], 0.5 mM; CATR 3.5 mM

sample_3: yAAC3, [U-100% 15N], 0.5 mM; ADP 40 mM

sample_conditions_1: ionic strength: 0.025 M; pH: 6; pressure: 1 atm; temperature: 303.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

CCPN, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links: