BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 27312

Title: Partial solid-state NMR 1H, 13C, 15N resonance assignments of a perdeuterated back-exchanged seven-transmembrane helical protein Anabaena Sensory Rhodopsin   PubMed: 29572785

Authors: Bolton, David; Brown, Leonid; Ladizhansky, Vladimir

Citation: Bolton, David; Brown, Leonid; Ladizhansky, Vladimir. "Partial solid-state NMR 1H, 13C, 15N resonance assignments of a perdeuterated back-exchanged seven-transmembrane helical protein Anabaena Sensory Rhodopsin"  Biomol. NMR Assign. ., .-. (2018).

Assembly members:
ASR, polymer, 235 residues, Formula weight is not available

Natural source:   Common Name: Anabaena variabilis   Taxonomy ID: 264691   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Anabaena variabilis

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
ASR: MNLESLLHWIYVAGMTIGAL HFWSLSRNPRGVPQYEYLVA MFIPIWSGLAYMAMAIDQGK VEAAGQIAHYARYIDWMVTT PLLLLSLSWTAMQFIKKDWT LIGFLMSTQIVVITSGLIAD LSERDWVRYLWYICGVCAFL IILWGIWNPLRAKTRTQSSE LANLYDKLVTYFTVLWIGYP IVWIIGPSGFGWINQTIDTF LFCLLPFFSKVGFSFLDLHG LRNLNDSRQHHHHHH

Data sets:
Data typeCount
13C chemical shifts186
15N chemical shifts95
1H chemical shifts95

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ASR, chain 11
2ASR, chain 21
3ASR, chain 31

Entities:

Entity 1, ASR, chain 1 235 residues - Formula weight is not available

1   METASNLEUGLUSERLEULEUHISTRPILE
2   TYRVALALAGLYMETTHRILEGLYALALEU
3   HISPHETRPSERLEUSERARGASNPROARG
4   GLYVALPROGLNTYRGLUTYRLEUVALALA
5   METPHEILEPROILETRPSERGLYLEUALA
6   TYRMETALAMETALAILEASPGLNGLYLYS
7   VALGLUALAALAGLYGLNILEALAHISTYR
8   ALAARGTYRILEASPTRPMETVALTHRTHR
9   PROLEULEULEULEUSERLEUSERTRPTHR
10   ALAMETGLNPHEILELYSLYSASPTRPTHR
11   LEUILEGLYPHELEUMETSERTHRGLNILE
12   VALVALILETHRSERGLYLEUILEALAASP
13   LEUSERGLUARGASPTRPVALARGTYRLEU
14   TRPTYRILECYSGLYVALCYSALAPHELEU
15   ILEILELEUTRPGLYILETRPASNPROLEU
16   ARGALALYSTHRARGTHRGLNSERSERGLU
17   LEUALAASNLEUTYRASPLYSLEUVALTHR
18   TYRPHETHRVALLEUTRPILEGLYTYRPRO
19   ILEVALTRPILEILEGLYPROSERGLYPHE
20   GLYTRPILEASNGLNTHRILEASPTHRPHE
21   LEUPHECYSLEULEUPROPHEPHESERLYS
22   VALGLYPHESERPHELEUASPLEUHISGLY
23   LEUARGASNLEUASNASPSERARGGLNHIS
24   HISHISHISHISHIS

Samples:

UCND_ASR: Anabaena Sensory Rhodopsin, [U-100% 13C; U-100% 15N; U-80% 2H], 1 mg

RAP_ASR: Anabaena Sensory Rhodopsin, [U-100% 13C; U-100% 15N, 15% 2H], 1 mg

sample_conditions_1: pH: 9; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
3D CONHUCND_ASRisotropicsample_conditions_1
3D CANHUCND_ASRisotropicsample_conditions_1
3D (CO)CA(CO)NHUCND_ASRisotropicsample_conditions_1
3D CO(CA)NHUCND_ASRisotropicsample_conditions_1
3D CONHRAP_ASRisotropicsample_conditions_1
3D CANHRAP_ASRisotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Keller and Wuthrich - data analysis, processing

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links: