BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 27324

Title: C-terminal domain of Cdc37 co-chaperone, unfolded in the presence of 8M Urea.   PubMed: 29343704

Authors: Bachman, Ashleigh; Keramisanou, Dimitra; Kumar M. V., Vasantha; Gelis, Ioannis

Citation: Bachman, Ashleigh; Keramisanou, Dimitra; Xu, Wanping; Beebe, Kristin; Moses, Michael; Vasantha Kumar, M; Gray, Geoffrey; Noor, Radwan Ebna; van der Vaart, Arjan; Neckers, Len; Gelis, Ioannis. "Phosphorylation induced cochaperone unfolding promotes kinase recruitment and client class-specific Hsp90 phosphorylation"  Nat. Commun. 9, 265-265 (2018).

Assembly members:
Cdc37, polymer, 93 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Cdc37: GMGPGGLDPVEVYESLPEEL QKCFDVKDVQMLQDAISKMD PTDAKYHMQRCIDSGLWVPN SKASEAKEGEEAGPGDPLLE AVPKTGDEKDVSV

Data sets:
Data typeCount
13C chemical shifts163
15N chemical shifts75
1H chemical shifts75

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1C-terminal domain of Cdc371

Entities:

Entity 1, C-terminal domain of Cdc37 93 residues - Formula weight is not available

1   GLYMETGLYPROGLYGLYLEUASPPROVAL
2   GLUVALTYRGLUSERLEUPROGLUGLULEU
3   GLNLYSCYSPHEASPVALLYSASPVALGLN
4   METLEUGLNASPALAILESERLYSMETASP
5   PROTHRASPALALYSTYRHISMETGLNARG
6   CYSILEASPSERGLYLEUTRPVALPROASN
7   SERLYSALASERGLUALALYSGLUGLYGLU
8   GLUALAGLYPROGLYASPPROLEULEUGLU
9   ALAVALPROLYSTHRGLYASPGLULYSASP
10   VALSERVAL

Samples:

sample_2: Cdc37, [U-100% 13C; U-100% 15N], 0.5 mM

sample_1: Cdc37, [U-100% 15N], 0.5 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 7.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_2isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Agilent direct drive 800 MHz

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