BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 27426

Title: Chemical shift assignments for mutant Hs MBD2 intrinsically disordered region

Authors: Williams, David

Citation: Yu, Xiaofei; Azzo, Alexander; Bilinovich, Stephanie; Kurita, Ryo; Nakamura, Yukio; Williams, David; Ginder, Gordon. "Disruption of the MBD2-NuRD complex but not MBD3-NuRD induces high levels of expression in human adult erythroid cells"  Not known ., .-..

Assembly members:
MBD2(R286E-L287A), polymer, 121 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
MBD2(R286E-L287A): GSDLNTTLPIRQTASIFKQP VTKVTNHPSNKVKSDPQRMN EQPRQLFWEKEAQGLSASDV TEQIIKTMELPKGLQGVGPG SNDETLLSAVASALHTSSAP ITGQVSAAVEKNPAVWLNTS Q

Data sets:
Data typeCount
13C chemical shifts335
15N chemical shifts107
1H chemical shifts403

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MBD2 mutant IDR1

Entities:

Entity 1, MBD2 mutant IDR 121 residues - Formula weight is not available

1   GLYSERASPLEUASNTHRTHRLEUPROILE
2   ARGGLNTHRALASERILEPHELYSGLNPRO
3   VALTHRLYSVALTHRASNHISPROSERASN
4   LYSVALLYSSERASPPROGLNARGMETASN
5   GLUGLNPROARGGLNLEUPHETRPGLULYS
6   GLUALAGLNGLYLEUSERALASERASPVAL
7   THRGLUGLNILEILELYSTHRMETGLULEU
8   PROLYSGLYLEUGLNGLYVALGLYPROGLY
9   SERASNASPGLUTHRLEULEUSERALAVAL
10   ALASERALALEUHISTHRSERSERALAPRO
11   ILETHRGLYGLNVALSERALAALAVALGLU
12   LYSASNPROALAVALTRPLEUASNTHRSER
13   GLN

Samples:

sample_1: MBD2 mutant IDR, [U-99% 13C; U-99% 15N], 1 mM; sodium phosphate 10 mM

sample_conditions_1: ionic strength: 0.03 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CcpNMR, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker INOVA 700 MHz

Related Database Links: