BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 27437

Title: 1H, 13CA, 13CB and 15N chemical shift assignments of b2-microglobulin and a-chain of the neonatal Fc receptor

Authors: Stoeppler, Daniel; Oschkinat, Hartmut

Citation: Stoeppler, Daniel; Macpherson, Alex; Smith-Penzel, Susanne; Basse, Nicolas; Lecomte, Fabien; Deboves, Herv; Taylor, Richard; Norman, Tim; Porter, John; Waters, Lorna; Westwood, Marta; Cossins, Ben; Cain, Katharine; White, James; Griffin, Robert; Prosser, Christine; Kelm, Sebastian; Sullivan, Amy; Fox III, David; Carr, Mark; Henry, Alistair; Taylor, Richard; Meier, Beat; Oschkinat, Hartmut; Lawson, Alastair. "Insight into Small Molecule Binding to the Neonatal Fc Receptor by X-ray Crystallography and 100 kHz Magic-Angle-Spinning NMR"  PLOS Biology ., .-..

Assembly members:
a-chain, polymer, 274 residues, Formula weight is not available
b2-microglobulin, polymer, 99 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Mammalian HEK293 cells

Entity Sequences (FASTA):
a-chain: AESHLSLLYHLTAVSSPAPG TPAFWVSGWLGPQQYLSYNS LRGEAEPCGAWVWENQVSWY WEKETTDLRIKEKLFLEAFK ALGGKGPYTLQGLLGCELGP DNTSVPTAKFALNGEEFMNF DLKQGTWGGDWPEALAISQR WQQQDKAANKELTFLLFSCP HRLREHLERGRGNLEWKEPP SMRLKARPSSPGFSVLTCSA FSFYPPELQLRFLRNGLAAG TGQGDFGPNSDGSFHASSSL TVKSGDEHHYCCIVQHAGLA QPLRVELESPAKSS
b2-microglobulin: IQRTPKIQVYSRHPAENGKS NFLNCYVSGFHPSDIEVDLL KNGERIEKVEHSDLSFSKDW SFYLLYYTEFTPTEKDEYAC RVNHVTLSQPKIVKWDRDM

Data sets:
Data typeCount
13C chemical shifts173
15N chemical shifts89
1H chemical shifts89

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1a-chain1
2b2-microglobulin2

Entities:

Entity 1, a-chain 274 residues - Formula weight is not available

1   ALAGLUSERHISLEUSERLEULEUTYRHIS
2   LEUTHRALAVALSERSERPROALAPROGLY
3   THRPROALAPHETRPVALSERGLYTRPLEU
4   GLYPROGLNGLNTYRLEUSERTYRASNSER
5   LEUARGGLYGLUALAGLUPROCYSGLYALA
6   TRPVALTRPGLUASNGLNVALSERTRPTYR
7   TRPGLULYSGLUTHRTHRASPLEUARGILE
8   LYSGLULYSLEUPHELEUGLUALAPHELYS
9   ALALEUGLYGLYLYSGLYPROTYRTHRLEU
10   GLNGLYLEULEUGLYCYSGLULEUGLYPRO
11   ASPASNTHRSERVALPROTHRALALYSPHE
12   ALALEUASNGLYGLUGLUPHEMETASNPHE
13   ASPLEULYSGLNGLYTHRTRPGLYGLYASP
14   TRPPROGLUALALEUALAILESERGLNARG
15   TRPGLNGLNGLNASPLYSALAALAASNLYS
16   GLULEUTHRPHELEULEUPHESERCYSPRO
17   HISARGLEUARGGLUHISLEUGLUARGGLY
18   ARGGLYASNLEUGLUTRPLYSGLUPROPRO
19   SERMETARGLEULYSALAARGPROSERSER
20   PROGLYPHESERVALLEUTHRCYSSERALA
21   PHESERPHETYRPROPROGLULEUGLNLEU
22   ARGPHELEUARGASNGLYLEUALAALAGLY
23   THRGLYGLNGLYASPPHEGLYPROASNSER
24   ASPGLYSERPHEHISALASERSERSERLEU
25   THRVALLYSSERGLYASPGLUHISHISTYR
26   CYSCYSILEVALGLNHISALAGLYLEUALA
27   GLNPROLEUARGVALGLULEUGLUSERPRO
28   ALALYSSERSER

Entity 2, b2-microglobulin 99 residues - Formula weight is not available

1   ILEGLNARGTHRPROLYSILEGLNVALTYR
2   SERARGHISPROALAGLUASNGLYLYSSER
3   ASNPHELEUASNCYSTYRVALSERGLYPHE
4   HISPROSERASPILEGLUVALASPLEULEU
5   LYSASNGLYGLUARGILEGLULYSVALGLU
6   HISSERASPLEUSERPHESERLYSASPTRP
7   SERPHETYRLEULEUTYRTYRTHRGLUPHE
8   THRPROTHRGLULYSASPGLUTYRALACYS
9   ARGVALASNHISVALTHRLEUSERGLNPRO
10   LYSILEVALLYSTRPASPARGASPMET

Samples:

sample_1: a-chain, [U-100% 13C; U-100% 15N], 550 uM; b2-microglobulin, [U-100% 13C; U-100% 15N], 550 uM; NaCl 100 mM

sample_conditions_1: ionic strength: 100 mM; pH: 7.4; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

CCPN, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 850 MHz

Related Database Links: