BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 30076

Title: Domain 4 Segment 6 of voltage-gated sodium channel Nav1.4

Authors: Niitsu, Ai; Egawa, Ayako; Ikeda, Keisuke; Tachibana, Kazuo; Fujiwara, Toshimichi

Citation: Niitsu, Ai; Egawa, Ayako; Ikeda, Keisuke; Tachibana, Kazuo; Fujiwara, Toshimichi. "Veratridine binding to a transmembrane segment of mammalian sodium channel Nav1.4 determined by solid-state NMR"  . ., .-..

Assembly members:
Sodium channel protein type 4 subunit alpha, polymer, 35 residues, 4033.070 Da.

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
Sodium channel protein type 4 subunit alpha: KKKGICFFCSYIIISFLIVV NMYIAIILENFKKKX

Data sets:
Data typeCount
13C chemical shifts111

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 35 residues - 4033.070 Da.

1   LYSLYSLYSGLYILECYSPHEPHECYSSER
2   TYRILEILEILESERPHELEUILEVALVAL
3   ASNMETTYRILEALAILEILELEUGLUASN
4   PHELYSLYSLYSNH2

Samples:

sample_1: DMPC, d-54, 69%; peptide, U-13C I23, L17, V20, 16%

sample_2: DMPC, d-54, 69%; peptide, U-13C G, I, F, L, V, A, 16%

sample_conditions_1: ionic strength: 0 mM; pH: 7.4; pressure: 1 atm; temperature: 213 K

Experiments:

NameSampleSample stateSample conditions
13C-13C dipolar-assisted rotational resonancesample_1anisotropicsample_conditions_1
13C-13C dipolar-assisted rotational resonancesample_2anisotropicsample_conditions_1
13C observation of 2H-selective 1H-depolarizationsample_1anisotropicsample_conditions_1

Software:

CHARMM, M. KARPLUS ET AL, HARVARD UNIVERSITY - refinement

DELTA - structure solution

FELIX - structure solution

SPARKY - structure solution

NMR spectrometers:

  • Varian Infinity Plus 700 700 MHz

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