BMRB Entry 30147

Title:
Notch1 transmembrane and associated juxtamembrane segment
Deposition date:
2016-07-25
Original release date:
2017-05-04
Authors:
Deatherage, C.; Lu, Z.; Kroncke, B.
Citation:

Citation: Deatherage, Catherine; Lu, Zhenwei; Kroncke, Brett; Ma, Sirui; Smith, Jarrod; Voehler, Markus; McFeeters, Robert; Sanders, Charles. "Structural and biochemical differences between the Notch and the amyloid precursor protein transmembrane domains"  Sci. Adv. 3, e1602794-e1602794 (2017).
PubMed: 28439555

Assembly members:

Assembly members:
entity_1, polymer, 59 residues, 6849.948 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts137
15N chemical shifts46
1H chemical shifts45

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 59 residues - 6849.948 Da.

1   METGLYHISHISHISHISHISHISVALGLN
2   SERGLUTHRVALGLUPROPROPROPROALA
3   GLNLEUHISPHEMETTYRVALALAALAALA
4   ALAPHEVALLEULEUPHEPHEVALGLYCYS
5   GLYVALLEULEUSERARGLYSARGARGARG
6   GLNHISGLYGLNLEUTRPPHEPROGLU

Samples:

sample_1: DHPC/DMPC bicelle 150 mg/mL; DTT 2 mM; EDTA 1 mM; Notch1, [U-99% 15N], 350 uM; imidazole 65 mM

sample_2: DHPC/DMPC bicelle 150 mg/mL; Notch1, [U-99% 13C; U-99% 15N], 500 uM

sample_3: DHPC/DMPC 150 mg/mL; DTT 2 mM; EDTA 1 mM; Notch1, [U-99% 13C; U-99% 15N], 500 uM; imidazole 65 mM

sample_conditions_1: ionic strength: 65 mM; pH: 5.5; pressure: 1 atm; temperature: 318 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D C(CO)NHsample_3isotropicsample_conditions_1
3D H(CCO)NHsample_3isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment, peak picking

TALOS, Cornilescu, Delaglio and Bax - chemical shift calculation

X-PLOR, Brunger - refinement

NMR spectrometers:

  • Bruker AvanceIII 900 MHz
  • Bruker AvanceIII 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks