BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 30220

Title: Solution NMR Structure of NERD-C, a natively folded tetramutant of the B1 domain of streptococcal protein G (GB1)

Authors: Damry, A.; Davey, J.; Goto, N.; Chica, R.

Citation: Davey, J.; Damry, A.; Goto, N.; Chica, R.. "Computational Design of Dynamics into a Stable Globular Protein"  To be published ., .-..

Assembly members:
entity_1, polymer, 56 residues, 6165.795 Da.

Natural source:   Common Name: Streptococcus sp. GX7805   Taxonomy ID: 1325   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptococcus Streptococcus sp. GX7805

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity_1: MTFKLIINGKTLKGETTTEA VDAATAEKVLKQYANDNGID GEWTYDDATKTFTVTE

Data typeCount
13C chemical shifts387
15N chemical shifts61
1H chemical shifts635

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 56 residues - 6165.795 Da.

1   METTHRPHELYSLEUILEILEASNGLYLYS
2   THRLEULYSGLYGLUTHRTHRTHRGLUALA
3   VALASPALAALATHRALAGLULYSVALLEU
4   LYSGLNTYRALAASNASPASNGLYILEASP
5   GLYGLUTRPTHRTYRASPASPALATHRLYS
6   THRPHETHRVALTHRGLU

Samples:

sample_1: protein (GB1), [U-98% 15N], 1.0 mM; sodium phosphate 10 mM

sample_2: protein (GB1), [U-99% 13C; U-98% 15N], 1.0 mM; sodium phosphate 10 mM

sample_3: protein (GB1), [U-99% 13C; U-98% 15N], 1.0 mM; sodium phosphate 10 mM

sample_conditions_1: ionic strength: 10 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_3isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_3isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D CCH-TOCSYsample_3isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HCCH-COSYsample_3isotropicsample_conditions_1
3D HCCH-TOCSYsample_3isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
2D 1H-13C HSQCsample_3isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - refinement, structure calculation

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking

VNMR, Varian - collection

NMR spectrometers:

  • Varian Inova 500 MHz

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