BMRB Entry 30632

Title:
Structural Basis for Client Recognition and Activity of Hsp40 Chaperones
Deposition date:
2019-07-09
Original release date:
2019-09-11
Authors:
Jiang, Y.; Rossi, P.; Kalodimos, C.
Citation:

Citation: Jiang, Y.; Rossi, P.; Kalodimos, C.. "Structural basis for client recognition and activity of Hsp40 chaperones"  Science 365, 1313-1319 (2019).
PubMed: 31604242

Assembly members:

Assembly members:
entity_1, polymer, 94 residues, 9571.893 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 83333   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)

Data sets:
Data typeCount
13C chemical shifts354
15N chemical shifts79
1H chemical shifts516

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 94 residues - 9571.893 Da.

1   METLYSASPGLYALAVALMETVALMETSER
2   TYRGLYASNSERGLUGLUASPGLYSERGLY
3   GLYSERGLYGLYSERGLYGLYSERGLNASP
4   LEUTYRALATHRLEUASPVALPROALAPRO
5   ILEALAVALVALGLYGLYLYSVALARGALA
6   METTHRLEUGLUGLYPROVALGLUVALALA
7   VALPROPROARGTHRGLNALAGLYARGLYS
8   LEUARGLEULYSGLYLYSGLYPHEPROGLY
9   PROALAGLYARGGLYASPLEUTYRLEUGLU
10   VALARGILETHR

Samples:

sample_1: PhoA-Hsp40/DnaJ CBD2 fusion, [U-100% 13C; U-100% 15N], 1 mM; potassium chloride 75 mM; potassium phosphate 20 mM; sodium azide 0.04%

sample_conditions_1: ionic strength: 100 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D CCH TOCSYsample_1isotropicsample_conditions_1
3D CCH NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

PINE, Bahrami, Markley, Assadi, and Eghbalnia - chemical shift assignment

Sparky, Goddard - peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TopSpin v4.0, Bruker Biospin - collection

PSVS, Bhattacharya and Montelione - data analysis

NMR spectrometers:

  • Bruker AVANCE NEO 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks