BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 3322

Title: Characterisation of a cellulosome dockerin domain from the anaerobic fungus Piromyces equi   PubMed: 11524680

Authors: Raghothama, Srinivasrao; Eberhardt, Ruth; Simpson, Peter; Wigelsworth, Darran; White, Peter; Hazlewood, Geoffrey; Nagy, Tibor; Gilbert, Harry; Williamson, Michael

Citation: Raghothama, Srinivasrao; Eberhardt, Ruth; Simpson, Peter; Wigelsworth, Darran; White, Peter; Hazlewood, Geoffrey; Nagy, Tibor; Gilbert, Harry; Williamson, Michael. "Characterisation of a cellulosome dockerin domain from the anaerobic fungus Piromyces equi"  Nat. Struct. Biol. 8, 775-778 (2001).

Assembly members:
dockerin, polymer, 52 residues, Formula weight is not available

Natural source:   Common Name: Piromyces equi   Taxonomy ID: 99929   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Piromyces equi

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
dockerin: GSCWAQSQGYNCCNNPSSTK VEYTDASGQWGVQNGQWCGI DYSYGQNQGNES

Data sets:
  • assigned_chemical_shifts
Data typeCount
15N chemical shifts62
1H chemical shifts301

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1dockerin1

Entities:

Entity 1, dockerin 52 residues - Formula weight is not available

1   GLYSERCYSTRPALAGLNSERGLNGLYTYR
2   ASNCYSCYSASNASNPROSERSERTHRLYS
3   VALGLUTYRTHRASPALASERGLYGLNTRP
4   GLYVALGLNASNGLYGLNTRPCYSGLYILE
5   ASPTYRSERTYRGLYGLNASNGLNGLYASN
6   GLUSER

Samples:

Sample_1: dockerin, [U-15N], 1 mM

condition_1: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D-HSQC/NOESYSample_1not availablecondition_1
HNHASample_1not availablecondition_1
HNHBSample_1not availablecondition_1
2D-HSQCSample_1not availablecondition_1
15N decoupled NOESYSample_1not availablecondition_1
TOCSYSample_1not availablecondition_1
DQF-COSYSample_1not availablecondition_1
E.COSYSample_1not availablecondition_1

Software:

FELIX -

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker DRX 600 MHz

Related Database Links:

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