BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 34124

Title: Solution structure of Drosophila melanogaster Loquacious dsRBD1

Authors: Tants, J.N.; Fesser, S.; Kern, T.; Stehle, R.; Geerlof, A.; Wunderlich, C.; Hartlmuller, C.; Boettcher, R.; Kunzelmann, S.; Lange, O.; Kreutz, C.; Foerstemann, K.; Sattler, M.

Citation: Tants, J.N.; Fesser, S.; Kern, T.; Stehle, R.; Geerlof, A.; Wunderlich, C.; Boettcher, R.; Kunzelmann, S.; Lange, O.; Kreutz, C.; Foerstemann, K.; Sattler, M.. "Molecular basis for asymmetry sensing of siRNAs by the Drosophila Loqs-PD / Dcr-2 complex in RNA interference"  Nucleic Acids Res. ., .-. (2017).

Assembly members:
entity_1, polymer, 83 residues, 9105.568 Da.

Natural source:   Common Name: Fruit fly   Taxonomy ID: 7227   Superkingdom: not available   Kingdom: not available   Genus/species: Drosophila melanogaster

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity_1: NGLAMKTPVSILQELLSRRG ITPGYELVQIEGAIHEPTFR FRVSFKDKDTPFTAMGAGRS KKEAKHAAARALIDKLIGAQ LPE

Data sets:
Data typeCount
13C chemical shifts237
15N chemical shifts76
1H chemical shifts457

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 83 residues - 9105.568 Da.

1   ASNGLYLEUALAMETLYSTHRPROVALSER
2   ILELEUGLNGLULEULEUSERARGARGGLY
3   ILETHRPROGLYTYRGLULEUVALGLNILE
4   GLUGLYALAILEHISGLUPROTHRPHEARG
5   PHEARGVALSERPHELYSASPLYSASPTHR
6   PROPHETHRALAMETGLYALAGLYARGSER
7   LYSLYSGLUALALYSHISALAALAALAARG
8   ALALEUILEASPLYSLEUILEGLYALAGLN
9   LEUPROGLU

Samples:

sample_1: dsRBD1, [U-13C; U-15N], 2 g/L; NaCl 500 mM; PO4 buffer 20 mM

sample_conditions_1: ionic strength: 520 mM; pH: 6.5; pressure: 101325 Pa; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

CS-Rosetta, Shen, Vernon, Baker and Bax - structure calculation

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker AvanceIII 900 MHz

Related Database Links: