BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 34171

Title: NMR derived model of the 5'-splice site of SMN2 in complex with the 5'-end of U1 snRNA

Authors: Campagne, S.; Clery, A.; Allain, F.

Citation: Sivaramakrishnan, M.; McCarthy, K.; Campagne, S.; Huber, S.; Meier, S.; Augustin, A.; Heckel, T.; Meistermann, H.; Hug, M.; Birrer, P.; Moursy, A.; Khawaja, S.; Schmucki, R.; Berntenis, N.; Giroud, N.; Golling, S.; Tzouros, N.; Banfai, B.; Duran-Pacheco, G.; Lamerz, J.; Hsiu Liu, Y.; Luebbers, T.; Ratni, A.; Ebeling, M.; Clery, A.; Paushkin, S.; Krainer, A.; Allain, F.; Metzger, F.. "Binding to SMN2 pre-mRNA-Protein complex elicits specificity for small molecule splicing modifiers"  to be published ., .-..

Assembly members:
entity_1, polymer, 11 residues, 3428.074 Da.
entity_2, polymer, 11 residues, 3547.162 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Homo sapiens

Entity Sequences (FASTA):
entity_1: AUACXXACCUG
entity_2: GGAGUAAGUCU

Data sets:
Data typeCount
13C chemical shifts147
1H chemical shifts166

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_22

Entities:

Entity 1, entity_1 11 residues - 3428.074 Da.

1   AUACPSUPSUACCU
2   G

Entity 2, entity_2 11 residues - 3547.162 Da.

1   GGAGUAAGUC
2   U

Samples:

sample_1: MES, deuterated, 10 mM; RNA (5'-R(P*AP*UP*AP*CP*(PSU)P*(PSU)P*AP*CP*CP*UP*G)-3') 1 mM; RNA (5'-R(P*GP*GP*AP*GP*UP*AP*AP*GP*UP*CP*U)-3') 1 mM; sodium chloride 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 5.5; pressure: 1 bar; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment

CYANA v3.98, Guntert, Mumenthaler and Wuthrich - structure calculation

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker AvanceIII 900 MHz

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