BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4032

Title: 1H, 13C and 15N Resonance Assignments for the Bovine Pancreatic [C65S, C72S] Ribonuclease A at pH* 4.6 and Temperature of 20 deg. C   PubMed: 9188686

Authors: Shimotakahara, Sakurako; Rios, Carlos; Laity, John; Zimmerman, Diane; Scheraga, Harold; Montelione, Gaetano

Citation: Shimotakahara, Sakurako; Rios, Carlos; Laity, John; Zimmerman, Diane; Scheraga, Harold; Montelione, Gaetano. "NMR Structural Analysis of an Analog of an Intermediate Formed in the Rate-Determining Step of One Pathway in the Oxidative Folding of Bovine Pancreatic Ribonuclease A: Automated Analysis of 1H, 13C and 15N Resonance Assignments for Wild-Type and [C65S, C72S] Mutant Forms."  Biochemistry 36, 6915-6929 (1997).

Assembly members:
bovine pancreatic [C65S,C72S] ribonuclease A, polymer, 124 residues, Formula weight is not available

Natural source:   Common Name: 9913   Taxonomy ID: Eukaryota   Superkingdom: Metazoa   Kingdom: not available   Genus/species: Bos taurus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
bovine pancreatic [C65S,C72S] ribonuclease A: KETAAAKFERQHMDSSTSAA SSSNYCNQMMKSRNLTKDRC KPVNTFVHESLADVQAVCSQ KNVASKNGQTNSYQSYSTMS ITDCRETGSSKYPNCAYKTT QANKHIIVACEGNPYVPVHF DASV

Data sets:
Data typeCount
13C chemical shifts363
15N chemical shifts119
1H chemical shifts244

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1bovine pancreatic [C65S,C72S] ribonuclease A1

Entities:

Entity 1, bovine pancreatic [C65S,C72S] ribonuclease A 124 residues - Formula weight is not available

1   LYSGLUTHRALAALAALALYSPHEGLUARG
2   GLNHISMETASPSERSERTHRSERALAALA
3   SERSERSERASNTYRCYSASNGLNMETMET
4   LYSSERARGASNLEUTHRLYSASPARGCYS
5   LYSPROVALASNTHRPHEVALHISGLUSER
6   LEUALAASPVALGLNALAVALCYSSERGLN
7   LYSASNVALALASERLYSASNGLYGLNTHR
8   ASNSERTYRGLNSERTYRSERTHRMETSER
9   ILETHRASPCYSARGGLUTHRGLYSERSER
10   LYSTYRPROASNCYSALATYRLYSTHRTHR
11   GLNALAASNLYSHISILEILEVALALACYS
12   GLUGLYASNPROTYRVALPROVALHISPHE
13   ASPALASERVAL

Samples:

sample_one: bovine pancreatic [C65S,C72S] ribonuclease A, [U-15N], 2.7 mM; D2O 10%; H2O 90%; NaCl 0.0 mM; KCl 0.0 mM

sample_two: bovine pancreatic [C65S,C72S] ribonuclease A, [U-15N;U-13C], 3.0 mM; D2O 10%; H2O 90%

sample_conditions_one: pH*: 4.6; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

BMRB 1072 16010 16011 16503 16742 17099 17172 19065 2928 385 4031 443
PDB
EMBL CAA30263 CAA33801 CAB37066
GB AAA72269 AAA72757 AAB35594 AAB36134 AAI49530
PIR JC5560 NRBOB
PRF 630436A
REF NP_001014408 XP_005211519 XP_005901936 XP_010837737
SP P61823 P61824
TPE CDG32088
TPG DAA25470