BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4062

Title: 1H and 15N Resonance Assignments and Secondary Structure of the Carbon Monoxide Complex of Sperm Whale Myoglobin

Authors: Theriault, Yves; Pochapsky, Thomas; Dalvit, Claudio; Chiu, Mark; Sligar, Stephen; Wright, Peter

Citation: Theriault, Yves; Pochapsky, Thomas; Dalvit, Claudio; Chiu, Mark; Sligar, Stephen; Wright, Peter. "1H and 15N Resonance Assignments and Secondary Structure of the Carbon Monoxide Complex of Sperm Whale Myoglobin"  J. Biomol. NMR 4, 491-504 (1994).

Assembly members:
apomyoglobin, polymer, 154 residues, Formula weight is not available
PROTOPORPHYRIN IX, non-polymer, 562.658 Da.
CARBON MONOXIDE, non-polymer, 28.010 Da.

Natural source:   Common Name: sperm whale   Taxonomy ID: 9755   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Physeter catodon

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
apomyoglobin: MVLSEGEWQLVLHVWAKVEA DVAGHGQDILIRLFKSHPET LEKFDRFKHLKTEAEMKASE DLKKHGVTVLTALGAILKKK GHHEAELKPLAQSHATKHKI PIKYLEFISEAIIHVLHSRH PGDFGADAQGAMNKALELFR KDIAAKYKELGYQG

Data sets:
Data typeCount
15N chemical shifts148
1H chemical shifts850

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1apomyoglobin1
2Iron protoporphyrin IX2
3Carbon monoxide3

Entities:

Entity 1, apomyoglobin 154 residues - Formula weight is not available

1   METVALLEUSERGLUGLYGLUTRPGLNLEU
2   VALLEUHISVALTRPALALYSVALGLUALA
3   ASPVALALAGLYHISGLYGLNASPILELEU
4   ILEARGLEUPHELYSSERHISPROGLUTHR
5   LEUGLULYSPHEASPARGPHELYSHISLEU
6   LYSTHRGLUALAGLUMETLYSALASERGLU
7   ASPLEULYSLYSHISGLYVALTHRVALLEU
8   THRALALEUGLYALAILELEULYSLYSLYS
9   GLYHISHISGLUALAGLULEULYSPROLEU
10   ALAGLNSERHISALATHRLYSHISLYSILE
11   PROILELYSTYRLEUGLUPHEILESERGLU
12   ALAILEILEHISVALLEUHISSERARGHIS
13   PROGLYASPPHEGLYALAASPALAGLNGLY
14   ALAMETASNLYSALALEUGLULEUPHEARG
15   LYSASPILEALAALALYSTYRLYSGLULEU
16   GLYTYRGLNGLY

Entity 2, Iron protoporphyrin IX - C34 H34 N4 O4 - 562.658 Da.

1   PP9

Entity 3, Carbon monoxide - C O - 28.010 Da.

1   CMO

Samples:

sample_one: myoglobin carbon monoxide complex, [U-15N], mM; potassium phosphate 0.05 mM; D20 100%

sample_two: myoglobin carbon monoxide complex, [U-15N], mM; potassium phosphate 0.05 mM; D20 10%; H2O 90%

experimental_conditions_MbCO: pH: 5.6; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
not availablenot availablenot availableexperimental_conditions_MbCO

Software:

No software information available

NMR spectrometers:

  • Bruker AM-500 500 MHz
  • Bruker AMX-600 600MHz MHz

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