BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4089

Title: Peptide Deformylase Catalytic Core (Residues 1 - 147)

Authors: MEINNEL, T.; DARDEL, F.

Citation: DARDEL, F.; RAGUSA, S.; LAZENNEC, C.; BLANQUET, S.; MEINNEL, T.. "Solution Structure of Nickel-peptide Deformylase"  J. Mol. Biol. 280, 501-513 (1998).

Assembly members:
peptide deformylase, polymer, 147 residues, Formula weight is not available
NI, non-polymer, 58.693 Da.

Natural source:   Common Name: not available   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: ESCHERICHIA COLI

Experimental source:   Production method: recombinant technology   Host organism: PUC18 (IPTG-INDUCIBLE LAC PROMOTURCE 5 EXPRESSION_SYSTEM_PLASMID: PDEF-EC1-147

Entity Sequences (FASTA):
peptide deformylase: AVLQVLHIPDERLRKVAKPV EEVNAEIQRIVDDMFETMYA EEGIGLAATQVDIHQRIIVI DVSENRDERLVLINPELLEK SGETGIEEGCLSIPEQRALV PRAEKVKIRALDRDGKPFEL EADGLLAICIQHEMDHLVGK LFMDYLS

Data sets:
Data typeCount
13C chemical shifts487
15N chemical shifts160
1H chemical shifts1110

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1peptide deformylase1
2Ni2

Entities:

Entity 1, peptide deformylase 147 residues - Formula weight is not available

1   ALAVALLEUGLNVALLEUHISILEPROASP
2   GLUARGLEUARGLYSVALALALYSPROVAL
3   GLUGLUVALASNALAGLUILEGLNARGILE
4   VALASPASPMETPHEGLUTHRMETTYRALA
5   GLUGLUGLYILEGLYLEUALAALATHRGLN
6   VALASPILEHISGLNARGILEILEVALILE
7   ASPVALSERGLUASNARGASPGLUARGLEU
8   VALLEUILEASNPROGLULEULEUGLULYS
9   SERGLYGLUTHRGLYILEGLUGLUGLYCYS
10   LEUSERILEPROGLUGLNARGALALEUVAL
11   PROARGALAGLULYSVALLYSILEARGALA
12   LEUASPARGASPGLYLYSPROPHEGLULEU
13   GLUALAASPGLYLEULEUALAILECYSILE
14   GLNHISGLUMETASPHISLEUVALGLYLYS
15   LEUPHEMETASPTYRLEUSER

Entity 2, Ni - Ni - 58.693 Da.

1   NI

Samples:

sample_1: data not available

sample_cond_1: ionic strength: 20 mM; pH: 7.2 na; pressure: 1 atm; temperature: 318 K

Experiments:

NameSampleSample stateSample conditions
NOESYsample_1not availablesample_cond_1
DQF-COSYsample_1not availablesample_cond_1
CT-HSQCsample_1not availablesample_cond_1
HNCAsample_1not availablesample_cond_1
NOESY-HMQCsample_1not availablesample_cond_1
HCCH-TOCSYsample_1not availablesample_cond_1
HMBC-HSQC,HNHBsample_1not availablesample_cond_1

Software:

No software information available

NMR spectrometers:

  • Bruker AMX 600 MHz
  • Bruker DRX 600 MHz

Related Database Links:

PDB
DBJ BAB37575 BAE78005 BAG79085 BAH66202 BAI27558
EMBL CAA11508 CAA45206 CAA54367 CAA54826 CAD09179
GB AAA58084 AAC76312 AAF76758 AAG58408 AAL22269
PIR AB1010
REF NP_312179 NP_417745 NP_458493 NP_462310 NP_709074
SP A1AGH8 A8A591 A8AQI1 A9MN80 A9N8B1