BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4103

Title: 1H, 13C and 15N Chemical Shift Assignments of the 14mer DNA Duplex BS2

Authors: Fernandez, Cesar; Szyperski, Thomas; Ono, Akira; Iwai, Hideo; Tate, Shin-ichi; Kainosho, Masatsune; Wuthrich, Kurt

Citation: Fernandez, Cesar; Szyperski, Thomas; Ono, Akira; Iwai, Hideo; Tate, Shin-ichi; Kainosho, Masatsune; Wuthrich, Kurt. "NMR with 13C,15N-doubly-labeled DNA: the Antennapedia Homeodomain Complex with a 14mer DNA Duplex"  J. Biomol. NMR ., .-. (1998).

Assembly members:
BS2_strand_A, polymer, 14 residues, Formula weight is not available
BS2_strand_B, polymer, 14 residues, Formula weight is not available

Natural source:   Common Name: fruit fly   Taxonomy ID: not available   Superkingdom: Eukaryota   Kingdom: Animalia   Genus/species: Drosophila melanogaster

Experimental source:   Production method: Chemical synthesis

Entity Sequences (FASTA):
BS2_strand_A: GAAAGCCATTAGAG
BS2_strand_B: CTCTAATGGCTTTC

Data sets:
Data typeCount
13C chemical shifts188
15N chemical shifts22
1H chemical shifts258

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1BS2_strand_A1
2BS2_strand_B2

Entities:

Entity 1, BS2_strand_A 14 residues - Formula weight is not available

1   DGDADADADGDCDCDADTDT
2   DADGDADG

Entity 2, BS2_strand_B 14 residues - Formula weight is not available

1   DCDTDCDTDADADTDGDGDC
2   DTDTDTDC

Samples:

sample_one: 14mer DNA duplex containing the operator sequence BS2, [U-13C;U-15N], 1.0 mM; KPO4 50 mM; KCL 20 mM; D2O 10%; H2O 90%

sample_conditions_one: pH: 6.0; temperature: 309 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_onenot availablesample_conditions_one

Software:

No software information available

NMR spectrometers:

  • Bruker DRX500 500 MHz
  • Bruker DRX600 600 MHz
  • Varian Unity Plus Unity Plus 750 MHz

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