BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4106

Title: Solution Structure of the 30 Kda N-terminal Domain of Enzyme I of the Escherichia coli Phosphoenolpyruvate:Sugar Phosphotransferase System by Multidimensional NMR

Authors: Garrett, Daniel; Seok, Yeong-Jae; Liao, Der-Ing; Peterkofsky, Alan; Gronenborn, Angela; Clore, Marius

Citation: Garrett, Daniel; Seok, Yeong-Jae; Liao, Der-Ing; Peterkofsky, Alan; Gronenborn, Angela; Clore, Marius. "Solution Structure of the 30 Kda N-terminal Domain of Enzyme I of the Escherichia coli Phosphoenolpyruvate:Sugar Phosphotransferase System by Multidimensional NMR"  Biochemistry 36, 2517-2530 (1997).

Assembly members:
N-terminal Domain of Enzyme I, polymer, 259 residues, 28359 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
N-terminal Domain of Enzyme I: MISGILASPGIAFGKALLLK EDEIVIDRKKISADQVDQEV ERFLSGRAKASAQLETIKTK AGETFGEEKEAIFEGHIMLL EDEELEQEIIALIKDKHMTA DAAAHEVIEGQASALEELDD EYLKERAADVRDIGKRLLRN ILGLKIIDLSAIQDEVILVA ADLTPSETAQLNLKKVLGFI TDAGGRTSHTSIMARSLELP AIVGTGSVTSQVKNDDYLIL DAVNNQVYVNPTNEVIDKMR AVQEQVASEKAELAKLKDR

Data sets:
Data typeCount
13C chemical shifts769
15N chemical shifts29
1H chemical shifts1474

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1EIN1

Entities:

Entity 1, EIN 259 residues - 28359 Da.

1   METILESERGLYILELEUALASERPROGLY
2   ILEALAPHEGLYLYSALALEULEULEULYS
3   GLUASPGLUILEVALILEASPARGLYSLYS
4   ILESERALAASPGLNVALASPGLNGLUVAL
5   GLUARGPHELEUSERGLYARGALALYSALA
6   SERALAGLNLEUGLUTHRILELYSTHRLYS
7   ALAGLYGLUTHRPHEGLYGLUGLULYSGLU
8   ALAILEPHEGLUGLYHISILEMETLEULEU
9   GLUASPGLUGLULEUGLUGLNGLUILEILE
10   ALALEUILELYSASPLYSHISMETTHRALA
11   ASPALAALAALAHISGLUVALILEGLUGLY
12   GLNALASERALALEUGLUGLULEUASPASP
13   GLUTYRLEULYSGLUARGALAALAASPVAL
14   ARGASPILEGLYLYSARGLEULEUARGASN
15   ILELEUGLYLEULYSILEILEASPLEUSER
16   ALAILEGLNASPGLUVALILELEUVALALA
17   ALAASPLEUTHRPROSERGLUTHRALAGLN
18   LEUASNLEULYSLYSVALLEUGLYPHEILE
19   THRASPALAGLYGLYARGTHRSERHISTHR
20   SERILEMETALAARGSERLEUGLULEUPRO
21   ALAILEVALGLYTHRGLYSERVALTHRSER
22   GLNVALLYSASNASPASPTYRLEUILELEU
23   ASPALAVALASNASNGLNVALTYRVALASN
24   PROTHRASNGLUVALILEASPLYSMETARG
25   ALAVALGLNGLUGLNVALALASERGLULYS
26   ALAGLULEUALALYSLEULYSASPARG

Samples:

sample_DCN: N-terminal Domain of Enzyme I, [U-95% 2H; U-99% 13C; U-99% 15N], 1.4 – 1.5 mM

sample_HN: N-terminal Domain of Enzyme I, [U-100% 1H; U-100% 12C; U-99%1 5N], 1.4 – 1.5 mM

sample_DN: N-terminal Domain of Enzyme I, [U-100% 2H; U-100% 12C; U-99% 15N], 1.4 – 1.5 mM

sample_conditions_one: pH: 7.5; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
5 2D Exps (15N HSQC, 13C HSQC, HNCG, HN(CO)CG, HOHAHA)not availablenot availablenot available
17 3D Exps (too many to list, check paper)not availablenot availablenot available
3 4D Exps (13C,13C NOE, 13C,15N NOE, 15N,15N NOE)not availablenot availablenot available

Software:

PIPP v3.5.6 to 3.9.9 -

NMRPipe v1.x -

NMR spectrometers:

  • Bruker AMX 600 MHz
  • Bruker AMX 500 MHz

Related Database Links:

BMRB 17095 19958 25731 4264
PDB
DBJ BAA16290 BAB36711 BAG78231 BAI26669 BAI31701
EMBL CAP76888 CAQ32794 CAQ88296 CAQ99314 CAR03878
GB AAA24385 AAA24441 AAC75469 AAG57535 AAN43978
REF NP_311315 NP_416911 NP_708271 WP_000112669 WP_000328837
SP P08839