BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4114

Title: Chicken B-MYB DNA Binding Domain, Repeat 2 and Repeat 3   PubMed: 9657674

Authors: McIntosh, P.; Carr, M.; Wollborn, U.; Frenkiel, T.; Feeney, J.; McCormick, J.; Klempnauer, K.

Citation: McIntosh, P.; Carr, M.; Wollborn, U.; Frenkiel, T.; Feeney, J.; McCormick, J.; Klempnauer, K.. "Solution Structure of the B-MYB DNA-binding Domain: Evidence for a Key Role for Conformational Instability of the Protein in DNA-binding and Control of Gene Expression"  Biochemistry 37, 9619-9629 (1998).

Assembly members:
B-MYB, polymer, 110 residues, Formula weight is not available

Natural source:   Common Name: Chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
B-MYB: GIPDLVKGPWTKEEDQKVIE LVKKYGTKQWTLIAKHLKGR LGKQCRERWHNHLNPEVKKS SWTEEEDRIIFEAHKVLGNR WAEIAKLLPGRTDNAVKNHW NSTIKRKVDT

Data sets:
Data typeCount
13C chemical shifts348
15N chemical shifts127
1H chemical shifts827

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1B-MYB1

Entities:

Entity 1, B-MYB 110 residues - Formula weight is not available

1   GLYILEPROASPLEUVALLYSGLYPROTRP
2   THRLYSGLUGLUASPGLNLYSVALILEGLU
3   LEUVALLYSLYSTYRGLYTHRLYSGLNTRP
4   THRLEUILEALALYSHISLEULYSGLYARG
5   LEUGLYLYSGLNCYSARGGLUARGTRPHIS
6   ASNHISLEUASNPROGLUVALLYSLYSSER
7   SERTRPTHRGLUGLUGLUASPARGILEILE
8   PHEGLUALAHISLYSVALLEUGLYASNARG
9   TRPALAGLUILEALALYSLEULEUPROGLY
10   ARGTHRASPASNALAVALLYSASNHISTRP
11   ASNSERTHRILELYSARGLYSVALASPTHR

Samples:

sample_1: B-MYB mM

sample_cond_1: pH: 6.0; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available
2D 1H/13C HMQCnot availablenot availablenot available
2D F2 12C-FILTERED TOCSYnot availablenot availablenot available
3D 1H/13C HMQC-NOESYnot availablenot availablenot available
HCCH-COSYnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
3D 1H/15N NOESY-HSQCnot availablenot availablenot available
NOESY-HMQCnot availablenot availablenot available
HNHAnot availablenot availablenot available
HNHBnot availablenot availablenot available
HMQC-NOESY-HMQCnot availablenot availablenot available
ROESY-HMQCnot availablenot availablenot available
TOCSY-HMQCnot availablenot availablenot available
3D-1H/15N/13C CBCANHnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • VARIAN UNITY 600 MHz
  • VARIAN UNITY 500 MHz

Related Database Links:

PDB
DBJ BAC25979 BAG37322 BAH13925 BAI46683
EMBL CAA31655 CAA47839 CAA49898
GB AAH07585 AAH50842 AAH53555 AAI18124 AAP36828
REF NP_001068916 NP_001100006 NP_001231398 NP_001252784 NP_001265539
SP P10244 P48972 Q03237
TPG DAA23198