BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4116

Title: 1H, 13C and 15N Chemical Shift Assignments of the Colicin E9 Immunity Protein from Escherichia coli

Authors: Boetzel, R.; Czisch, M.; MacDonald, C.; Kaptein, R.; Hemmings, A.; James, Richard; Kleanthous, Colin; Moore, Geoffrey

Citation: Boetzel, R.; Czisch, M.; MacDonald, C.; Kaptein, R.; Hemmings, A.; James, Richard; Kleanthous, Colin; Moore, Geoffrey. "Assignment of 1H, 13C and 15N Signals of the Inhibitor Protein Im9 Bound to the DNase Domain of Colicin E9"  J. Biomol. NMR 12, 567-568 (1998).

Assembly members:
Im9 immunity protein, polymer, 86 residues, Formula weight is not available

Natural source:   Common Name: E.coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Im9 immunity protein: MELKHSISDYTEAEFLQLVT TICNADTSSEEELVKLVTHF EEMTEHPSGSDLIYYPKEGD DDSPSGIVNTVKQWRAANGK SGFKQG

Data sets:
Data typeCount
13C chemical shifts239
15N chemical shifts86
1H chemical shifts522

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1IM91

Entities:

Entity 1, IM9 86 residues - Formula weight is not available

1   METGLULEULYSHISSERILESERASPTYR
2   THRGLUALAGLUPHELEUGLNLEUVALTHR
3   THRILECYSASNALAASPTHRSERSERGLU
4   GLUGLULEUVALLYSLEUVALTHRHISPHE
5   GLUGLUMETTHRGLUHISPROSERGLYSER
6   ASPLEUILETYRTYRPROLYSGLUGLYASP
7   ASPASPSERPROSERGLYILEVALASNTHR
8   VALLYSGLNTRPARGALAALAASNGLYLYS
9   SERGLYPHELYSGLNGLY

Samples:

sample_one: Im9 immunity protein, [U-100%15N; U-100%13C], 4.0 mM; H2O 90%; D2O 10%

sample_conditions_one: pH: 6.2; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_onenot availablesample_conditions_one

Software:

No software information available

NMR spectrometers:

  • . . 500 MHz
  • . . 600 MHz

Related Database Links:

BMRB 4115
PDB
EMBL CAA33863
GB AAA23076 ACM07431
REF WP_012644887 YP_002533538
SP P13479