BMRB Entry 4124

Title:
Main-chain Signal Assignment for the PDZ2 Domain from Human Protein Tyrosine Phosphatase hPTP1E and its Complex with a C-terminal Peptide from the Fas Receptor
Deposition date:
1998-03-17
Original release date:
1999-02-02
Authors:
Ekiel, Irena; Banville, Denis; Shen, Shi Hsiang; Slon-Usakiewicz, Jacek; Koshy, Alex; Gehring, Kalle
Citation:

Citation: Ekiel, Irena; Banville, Denis; Shen, Shi Hsiang; Slon-Usakiewicz, Jacek; Koshy, Alex; Gehring, Kalle. "Main-chain Signal Assignment for the PDZ2Domain from Human Protein Tyrosine Phosphatase hPTP1E and its Complex with a C-terminal Peptide from the Fas Receptor"  J. Biomol. NMR 12, 455-456 (1998).

Assembly members:

Assembly members:
second PDZ domain from hPTP1E, polymer, 96 residues, 10006 Da.
C-terminal peptide from the Fas receptor, polymer, 15 residues, 1752 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
second PDZ domain from hPTP1E: PKPGDIFEVELAKNDNSLGI SVTGGVNTSVRHGGIYVKAV IPQGAAESDGRIHKGDRVLA VNGVSLEGATHKQAVETLRN TGQVVHLLLEKGQSPT
C-terminal peptide from the Fas receptor: DSENSNFRNEIQSLV

Data sets:
Data typeCount
15N chemical shifts88
1H chemical shifts88

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1PDZ21
2Fas_Cterm152

Entities:

Entity 1, PDZ2 96 residues - 10006 Da.

1   PROLYSPROGLYASPILEPHEGLUVALGLU
2   LEUALALYSASNASPASNSERLEUGLYILE
3   SERVALTHRGLYGLYVALASNTHRSERVAL
4   ARGHISGLYGLYILETYRVALLYSALAVAL
5   ILEPROGLNGLYALAALAGLUSERASPGLY
6   ARGILEHISLYSGLYASPARGVALLEUALA
7   VALASNGLYVALSERLEUGLUGLYALATHR
8   HISLYSGLNALAVALGLUTHRLEUARGASN
9   THRGLYGLNVALVALHISLEULEULEUGLU
10   LYSGLYGLNSERPROTHR

Entity 2, Fas_Cterm15 15 residues - 1752 Da.

1   ASPSERGLUASNSERASNPHEARGASNGLU
2   ILEGLNSERLEUVAL

Samples:

sample_one: hPTP1E-Fas receptor complex, [U-15N; U-13C], 3 mM; sodium phosphate buffer 50 mM; sodium chloride 150 mM; H2O 90%; D2O 10%

sample_conditions_one: pH: 6.9; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Bruker DRX500 500 MHz

Related Database Links:

BMRB 18833 18834 4123 4516
PDB
EMBL CAD48931 CAD48932

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks