BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4153

Title: High-Resolution Solution Structure of the Retinoid X Receptor DNA-Binding Domain

Authors: Holmbeck, Signe; Foster, M.; Casimiro, D.; Sem, D.; Dyson, H.; Wright, Peter

Citation: Holmbeck, Signe; Foster, M.; Casimiro, D.; Sem, D.; Dyson, H.; Wright, Peter. "High-Resolution Solution Structure of the Retinoid X Receptor DNA-Binding Domain"  J. Mol. Biol. 281, 271-284 (1998).

Assembly members:
RXR-alpha, polymer, 83 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
RXR-alpha: FTKHICAICGDRSSGKHYGV YSCEGCKGFFKRTVRKDLTY TCRDNKDCLIDKRQRNRCQY CRYQKALAMGMKREAVQEER QRX

Data sets:
Data typeCount
13C chemical shifts254
15N chemical shifts84
1H chemical shifts525

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RXR-DBD1
2Zn 12
3Zn 22

Entities:

Entity 1, RXR-DBD 83 residues - Formula weight is not available

1   PHETHRLYSHISILECYSALAILECYSGLY
2   ASPARGSERSERGLYLYSHISTYRGLYVAL
3   TYRSERCYSGLUGLYCYSLYSGLYPHEPHE
4   LYSARGTHRVALARGLYSASPLEUTHRTYR
5   THRCYSARGASPASNLYSASPCYSLEUILE
6   ASPLYSARGGLNARGASNARGCYSGLNTYR
7   CYSARGTYRGLNLYSALALEUALAMETGLY
8   METLYSARGGLUALAVALGLNGLUGLUARG
9   GLNARGNVA

Entity 2, Zn 1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_one: RXR-alpha 1.7 mM

sample_conditions_one: ionic strength: 0.12 M; pH: 6.6; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
CBCA(CO)NHsample_onenot availablesample_conditions_one
HBHA(CBCACO)NHsample_onenot availablesample_conditions_one
CBCANHsample_onenot availablesample_conditions_one
C(CO)NH-TOCSYsample_onenot availablesample_conditions_one
HCCH-COSYsample_onenot availablesample_conditions_one
HCCH-TOCSYsample_onenot availablesample_conditions_one
13C-EDITED NOESYsample_onenot availablesample_conditions_one
15N-EDITED NOESYsample_onenot availablesample_conditions_one
15N-EDITED TOCSYsample_onenot availablesample_conditions_one
HNHAsample_onenot availablesample_conditions_one
HNHBsample_onenot availablesample_conditions_one
HACAHB-COSYsample_onenot availablesample_conditions_one
13C-13COsample_onenot availablesample_conditions_one
13C-15N SPIN ECHO DIFFERENCE CT-HSQCsample_onenot availablesample_conditions_one

Software:

No software information available

NMR spectrometers:

  • Bruker AMX 500 MHz
  • Bruker DMX 600 MHz

Related Database Links:

PDB
DBJ BAE26004 BAE73032 BAG14373 BAG54745 BAG72733
EMBL CAA36982 CAA46962 CAL25727 CAL25728 CAL36079
GB AAA40080 AAA42093 AAB36777 AAB36778 AAC95154
PRF 1609194A
REF NP_001277410 NP_001277411 NP_001278849 NP_001278850 NP_001291272
SP P19793 P28700 Q05343
TPG DAA24096