BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4155

Title: Solution Structure of Eotaxin: A Chemokine that Selectively Recruits Eosinophils in Allergic Inflammation

Authors: Crump, M.; Rajarathnam, K.; Kim, K.-S; Sykes, B.

Citation: Crump, M.; Rajarathnam, K.; Kim, K.-S; Sykes, B.. "Solution Structure of Eotaxin: a Chemokine That Selectively Recruits Eosinophils in Allergic Inflammation"  J. Biol. Chem. 273, 22471-22479 (1998).

Assembly members:
Eotaxin, polymer, 74 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Eotaxin: XPASVPTTCCFNLANRKIPL QRLESYRRITSGKCPQKAVI FKTKLAKDICADPKKKWVQD SMKYLDQKSPTPKP

Data sets:
Data typeCount
15N chemical shifts64
1H chemical shifts478
coupling constants56

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1EOT1

Entities:

Entity 1, EOT 74 residues - Formula weight is not available

1   PCAPROALASERVALPROTHRTHRCYSCYS
2   PHEASNLEUALAASNARGLYSILEPROLEU
3   GLNARGLEUGLUSERTYRARGARGILETHR
4   SERGLYLYSCYSPROGLNLYSALAVALILE
5   PHELYSTHRLYSLEUALALYSASPILECYS
6   ALAASPPROLYSLYSLYSTRPVALGLNASP
7   SERMETLYSTYRLEUASPGLNLYSSERPRO
8   THRPROLYSPRO

Samples:

sample_one: Eotaxin, [U-15N], 1.0 mM; sodium azide 1.0 mM; sodium acetate 20.0 mM; DSS 1.0 mM

sample_conditions_one: pH: 5.0 na; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
DQF-COSYsample_onenot availablesample_conditions_one
TOCSYsample_onenot availablesample_conditions_one
NOESYsample_onenot availablesample_conditions_one
ROESYsample_onenot availablesample_conditions_one
13C-HSQC (natural abundance)sample_onenot availablesample_conditions_one
1H-15N HSQCnot available$sample_onenot available
7not available$sample_onenot available
8not available$sample_onenot available
9not available$sample_onenot available
10not available$sample_onenot available
11not available$sample_onenot available
12not available$sample_onenot available
not availablenot availablenot availablenot available

Software:

nmrPipe/PIPP v4.2 - Peak Picking of 15N edited TOCSY and 15N edited NOESY spectra

VNMR v5.3 Revision B - Peak Picking of COSY, NOESY and TOCSY spectra of unlabelled samples

NMR spectrometers:

  • Varian Unity 600 MHz
  • Varian Inova 500 MHz

Related Database Links:

BMRB 19989 4390
PDB
DBJ BAA08370 BAG72995
EMBL CAA99997 CAA99998 CAB07027 CAG33702
GB AAA98957 AAC50369 AAC51297 AAH17850 ABK41951
PRF 2208449A
REF NP_002977 XP_002827294 XP_003818054 XP_004041989 XP_004041990
SP P51671