BMRB Entry 4160
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4160
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Citation: Connolly, Kevin; Wojciak, Jonathan; Clubb, Robert. "Resonance Assignments of the Tn916 Integrase DNA-Binding Domain and Integrase:
DNA Complex" J. Biomol. NMR 14, 95-96 (1999).
Assembly members:
Tn916 integrase subunit, polymer, 81 residues, Formula weight is not available
Natural source: Common Name: Enterococcus faecalis Taxonomy ID: 1351 Superkingdom: Eubacteria Kingdom: not available Genus/species: Enterococcus faecalis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET11a
Entity Sequences (FASTA):
Tn916 integrase subunit: MSEKRRDNRGRILKTGESQR
KDGRYLYKYIDSFGEPQFVY
SWKLVATDRVPAGKRDCISL
REKIAELQKDIHDGIDVVGK
K
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 267 |
| 15N chemical shifts | 83 |
| 1H chemical shifts | 505 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TN916-int | 1 |
Entities:
Entity 1, TN916-int 81 residues - Formula weight is not available
| 1 | MET | SER | GLU | LYS | ARG | ARG | ASP | ASN | ARG | GLY | ||||
| 2 | ARG | ILE | LEU | LYS | THR | GLY | GLU | SER | GLN | ARG | ||||
| 3 | LYS | ASP | GLY | ARG | TYR | LEU | TYR | LYS | TYR | ILE | ||||
| 4 | ASP | SER | PHE | GLY | GLU | PRO | GLN | PHE | VAL | TYR | ||||
| 5 | SER | TRP | LYS | LEU | VAL | ALA | THR | ASP | ARG | VAL | ||||
| 6 | PRO | ALA | GLY | LYS | ARG | ASP | CYS | ILE | SER | LEU | ||||
| 7 | ARG | GLU | LYS | ILE | ALA | GLU | LEU | GLN | LYS | ASP | ||||
| 8 | ILE | HIS | ASP | GLY | ILE | ASP | VAL | VAL | GLY | LYS | ||||
| 9 | LYS |
Samples:
sample_one: Tn916 integrase subunit, [U-15N; U-13C], 1.3 mM
sample_conditions_one: pH: 6.0; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
No software information available
NMR spectrometers:
- unknown unknown 0 MHz
Related Database Links:
| BMRB | 4165 |
| PDB | |
| DBJ | BAG12510 BAK28444 BAN28951 GAD37680 |
| EMBL | CAA43360 CAO82956 CAP45546 CAQ49380 CAV31149 |
| GB | AAA19427 AAB60030 AAC36982 AAM99801 AAY63950 |
| PRF | 2114402Y |
| REF | NP_687929 WP_001291557 WP_001291559 WP_001291560 WP_001291561 |
| SP | P22886 P62904 P62905 |
| AlphaFold | P62905 P22886 P62904 |
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