BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4172

Title: Response Element of the Orphan Nuclear Receptor Rev-erb Beta

Authors: Castagne, C.; Terenzi, H.; Zakin, M.; Delepierre, M.

Citation: Castagne, C.; Terenzi, H.; Zakin, M.; Delepierre, M.. "Solution Structure of the Orphan Nuclear Receptor Rev-erb Beta Response Element by 1H, 31P NMR and Molecular Simulation"  Biochimie 82, 739-748 (2000).

Assembly members:
Rev-RE subunit 1, polymer, 15 residues, Formula weight is not available
Rev-RE subunit 2, polymer, 15 residues, Formula weight is not available

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
Rev-RE subunit 1: TAGAATGTAGGTCAG
Rev-RE subunit 2: CTGACCTACATTCTA

Data typeCount
1H chemical shifts268
31P chemical shifts28

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Rev-RE subunit 11
2Rev-RE subunit 22

Entities:

Entity 1, Rev-RE subunit 1 15 residues - Formula weight is not available

1   DTDADGDADADTDGDTDADG
2   DGDTDCDADG

Entity 2, Rev-RE subunit 2 15 residues - Formula weight is not available

1   DCDTDGDADCDCDTDADCDA
2   DTDTDCDTDA

Samples:

sample_one: Rev-RE subunit 1 3.6 mM; Rev-RE subunit 2 3.6 mM

sample_conditions_one: pH: 7.0; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
NOESY TOCSYsample_onenot availablesample_conditions_one
31P-1H hcosyinv and hsqc-tocsysample_onenot availablesample_conditions_one

Software:

FELIX v95.0 - cross-peaks volume calculation

JUMNA v8.0 - molecular simulation nucleic acid minimization

NMR spectrometers:

  • VARIAN UNITY 500 MHz

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