BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4187

Title: Nuclear Magnetic Resonance Structure of d(GCATATGATAG).d(CTATCATATGC): A Consensus Sequence for Promoters Recognized by Sigma-K RNA Polymerase

Authors: Tonelli, M.; Ragg, E.; Bianucci, A.; Lesiak, K.; James, T.

Citation: Tonelli, M.; Ragg, E.; Bianucci, A.; Lesiak, K.; James, T.. "Nuclear Magnetic Resonance Structure of d(GCATATGATAG).d(CTATCATATGC): A Consensus Sequence for Promoters Recognized by Sigma-K RNA Polymerase"  Biochemistry 37, 11745-11761 (1998).

Assembly members:
Promoter strand 1, polymer, 11 residues, Formula weight is not available
Promoter strand 2, polymer, 11 residues, Formula weight is not available

Natural source:   Common Name: Bacillus subtilis   Taxonomy ID: 1423   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
Promoter strand 1: GCATATGATAG
Promoter strand 2: CTATCATATGC

Data typeCount
1H chemical shifts204
coupling constants48

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DNA 11
2DNA 22

Entities:

Entity 1, DNA 1 11 residues - Formula weight is not available

1   DGDCDADTDADTDGDADTDA
2   DG

Entity 2, DNA 2 11 residues - Formula weight is not available

1   DCDTDADTDCDADTDADTDG
2   DC

Samples:

sample_one: Promoter strand 1 2.5 mM; Promoter strand 2 2.5 mM

sample_conditions_one: ionic strength: 0.5 M; pH: 7.0; pressure: 1 atm; temperature: 298.0 K

Experiments:

NameSampleSample stateSample conditions
NOESYsample_onenot availablesample_conditions_one
COSYsample_onenot availablesample_conditions_one

Software:

Striker - Spectral processing

SPARKY - Spectral assignment

AMBER - Structure calculation via molecular dynamic simulations

DNAminiCarlo - Structure calculation via Monte Carlo calculations

NMR spectrometers:

  • BRUKER AMX 600 MHz

Related Database Links:

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