BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4198

Title: Three-dimensional Structure of an Evolutionarily Conserved N-terminal Domain of Syntaxin 1A

Authors: Fernandez, Imma; Ubach, Josep; Dulubova, Irina; Zhang, Xiangyang; Sudhof, Thomas; Rizo, Josep

Citation: Fernandez, Imma; Ubach, Josep; Dulubova, Irina; Zhang, Xiangyang; Sudhof, Thomas; Rizo, Josep. "Three-dimensional Structure of an Evolutionarily Conserved N-terminal Domain of Syntaxin 1A"  Cell 94, 841-849 (1998).

Assembly members:
N-terminal domain of Syntaxin 1A, polymer, 161 residues, Formula weight is not available

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvergicus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
N-terminal domain of Syntaxin 1A: GSPGISGGGGGIPASMKDRT QELRTAKDSDDDDDVTVTVD RDRFMDEFFEQVEEIRGFID KIAENVEEVKRKHSAILASP NPDEKTKEELEELMSDIKKT ANKVRSKLKSIEQSIEQEEG LNRSSADLRIRKTQHSTLSR KFVEVMSEYNATQSDYRERS K

Data sets:
Data typeCount
13C chemical shifts518
15N chemical shifts128
1H chemical shifts858

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Nsyx1

Entities:

Entity 1, Nsyx 161 residues - Formula weight is not available

1   GLYSERPROGLYILESERGLYGLYGLYGLY
2   GLYILEPROALASERMETLYSASPARGTHR
3   GLNGLULEUARGTHRALALYSASPSERASP
4   ASPASPASPASPVALTHRVALTHRVALASP
5   ARGASPARGPHEMETASPGLUPHEPHEGLU
6   GLNVALGLUGLUILEARGGLYPHEILEASP
7   LYSILEALAGLUASNVALGLUGLUVALLYS
8   ARGLYSHISSERALAILELEUALASERPRO
9   ASNPROASPGLULYSTHRLYSGLUGLULEU
10   GLUGLULEUMETSERASPILELYSLYSTHR
11   ALAASNLYSVALARGSERLYSLEULYSSER
12   ILEGLUGLNSERILEGLUGLNGLUGLUGLY
13   LEUASNARGSERSERALAASPLEUARGILE
14   ARGLYSTHRGLNHISSERTHRLEUSERARG
15   LYSPHEVALGLUVALMETSERGLUTYRASN
16   ALATHRGLNSERASPTYRARGGLUARGSER
17   LYS

Samples:

sample1: N-terminal domain of Syntaxin 1A, [U-13C; U-15N], 1.0 mM

sample2: N-terminal domain of Syntaxin 1A, [U-15N], 1.0 mM

cond1: pH: 6.3; temperature: 305 K

Experiments:

NameSampleSample stateSample conditions
1N-15N TOCSY-HMQCnot availablenot availablenot available
1H-15N NOESY-HMQCnot availablenot availablenot available
HNCOnot availablenot availablenot available
HNCACBnot availablenot availablenot available
(H)CBCACO(CA)HAnot availablenot availablenot available
(H)C(CO)NH-TOCSYnot availablenot availablenot available
H(C)(CO)NH-TOCSYnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
3D 1H-15N NOESY-HSQCnot availablenot availablenot available
4D 1H-13C,1H-13C HMQC-NOESY-HMQCnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Varian UNITY 500 MHz

Related Database Links:

BMRB 15646
PDB
DBJ BAA01231 BAA02089 BAA07151 BAA28865 BAC78519
EMBL CAG33299 CAH93304
GB AAA20940 AAA42195 AAA53519 AAB22525 AAB65500
PIR G01485
PRF 2116295A
REF NP_001028037 NP_001126944 NP_001159375 NP_004594 NP_058081
SP O35526 P32850 P32851 Q16623 Q5R4L2