BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4199

Title: Determination of the Solution Structure of the N-Domain Plus Linker of Antarctic Eel Pout Antifreeze Protein RD3

Authors: Miura, Kazunori; Ohgiya, Satoru; Hoshino, Tamotsu; Nemoto, Nobuaki; Odaira, Masato; Nitta, Katsutoshi; Tsuda, Sakae

Citation: Miura, Kazunori; Ohgiya, Satoru; Hoshino, Tamotsu; Nemoto, Nobuaki; Odaira, Masato; Nitta, Katsutoshi; Tsuda, Sakae. "Determination of the Solution Structure of the N-Domain Plus Linker of Antarctic Eel Pout Antifreeze Protein RD3"  J. Biochem. 126, 387-394 (1999).

Assembly members:
N-term. domain with linker Type III Antifreeze protein RD3, polymer, 73 residues, Formula weight is not available

Natural source:   Common Name: antarctic eel pout   Taxonomy ID: 8201   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Lycodichthys dearborni

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
N-term. domain with linker Type III Antifreeze protein RD3: NKASVVANQLIPINTALTLI MMKAEVVTPMGIPAEEIPNL VGMQVNRAVPLGTTLMPDMV KNYEDGTTSPGLK

Data sets:
Data typeCount
15N chemical shifts74
1H chemical shifts525
coupling constants50

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RD3-N11

Entities:

Entity 1, RD3-N1 73 residues - Formula weight is not available

1   ASNLYSALASERVALVALALAASNGLNLEU
2   ILEPROILEASNTHRALALEUTHRLEUILE
3   METMETLYSALAGLUVALVALTHRPROMET
4   GLYILEPROALAGLUGLUILEPROASNLEU
5   VALGLYMETGLNVALASNARGALAVALPRO
6   LEUGLYTHRTHRLEUMETPROASPMETVAL
7   LYSASNTYRGLUASPGLYTHRTHRSERPRO
8   GLYLEULYS

Samples:

sample_one: N-term. domain with linker Type III Antifreeze protein RD3 1.0 mM

sample_two: N-term. domain with linker Type III Antifreeze protein RD3, [U-15N], 1.0 mM

sample_conditions: ionic strength: 0.05 M; pH: 6.7; temperature: 277 K

Experiments:

NameSampleSample stateSample conditions
DQFCOSYsample_twonot availablesample_conditions
TOCSYsample_twonot availablesample_conditions
NOESYsample_twonot availablesample_conditions
15N-HSQCsample_twonot availablesample_conditions
15N_TOCSYHSQCsample_twonot availablesample_conditions
15N-NOESYHSQCsample_twonot availablesample_conditions

Software:

No software information available

NMR spectrometers:

  • Varian Unity 500 MHz
  • JEOL JEOL 500 MHz

Related Database Links:

PDB
GB AFU88733 AFU88737 AFU88748
PRF 2109220A
SP P35751