BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4200

Title: NMR Solution Structure of the c-Myc-Max Heterodimeric Leucine Zipper

Authors: Lavigne, P.; Crump, M.; Gagne, S.; Hodges, R.; Kay, C.; Sykes, B.

Citation: Lavigne, P.; Crump, M.; Gagne, S.; Hodges, R.; Kay, C.; Sykes, B.. "Insights into the Mechanism of Heterodimerization from the 1H-NMR Solution Structure of the c-Myc-Max Heterodimeric Leucine Zipper"  J. Mol. Biol. 281, 165-181 (1998).

Assembly members:
Leucine Zipper domain of c-Myc, polymer, 34 residues, 3846 Da.
Max Leucine Zipper Domain, polymer, 34 residues, 3793 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
Leucine Zipper domain of c-Myc: XCGGVQAEEQKLISEEDLLR KRREQLKHKLEQLX
Max Leucine Zipper Domain: XCGGMRRKNDTHQQDIDDLK RQNALLEQQVRALX

Data typeCount
1H chemical shifts377

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1c-Myc LZ Chain A1
2Max LZ Chain B2

Entities:

Entity 1, c-Myc LZ Chain A 34 residues - 3846 Da.

1   ACECYSGLYGLYVALGLNALAGLUGLUGLN
2   LYSLEUILESERGLUGLUASPLEULEUARG
3   LYSARGARGGLUGLNLEULYSHISLYSLEU
4   GLUGLNLEUNH2

Entity 2, Max LZ Chain B 34 residues - 3793 Da.

1   ACECYSGLYGLYMETARGARGLYSASNASP
2   THRHISGLNGLNASPILEASPASPLEULYS
3   ARGGLNASNALALEULEUGLUGLNGLNVAL
4   ARGALALEUNH2

Samples:

sample_one: Leucine Zipper domain of c-Myc 1.0 mM; potassium phosphate 50 mM; H2O 90%; D2O 10%; KCl 50 mM; DSS 1 mM

sample_conditions: ionic strength: 0.1 M; pH: 4.8 na; temperature: 298.15 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_onenot availablesample_conditions

Software:

VNMR v5.2 -

NMR spectrometers:

  • Varian VXR 500 MHz
  • Varian Unity 600 MHz

Related Database Links:

PDB
DBJ BAA01374 BAA01375 BAG35731 BAG64849 BAI46221
EMBL CAA23831 CAA25015 CAA25106
GB AAA36340 AAA59880 AAA59881 AAA59882 AAA72907
PRF 0903253A 0907235A 1001205A 2122337A
REF NP_001136266 NP_002458 XP_003820503 XP_004047574 XP_008972161
SP A1YG22 P01106 P23583