BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4207

Title: CRO Repressor Insertion Mutant K56-[DGEVK]

Authors: Mossing, M.

Citation: Mossing, M.. "Solution Structure and Dynamics of a Designed Monomeric Variant of the Lambda CRO Repressor"  Protein Sci. 7, 983-993 (1998).

Assembly members:
CRO Repressor Insertion Mutant K56-[DGEVK], polymer, 71 residues, Formula weight is not available

Natural source:   Common Name: bacteriophage lambda   Taxonomy ID: 10710   Superkingdom: not available   Kingdom: not available   Genus/species: Lambda phage group bacteriophage lambda

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
CRO Repressor Insertion Mutant K56-[DGEVK]: MEQRITLKDYAMRFGQTKTA KDLGVYQSAINKAIHAGRKI FLTINADGSVYAEEVKDGEV KPFPSNKKTTA

Data sets:
Data typeCount
15N chemical shifts77
1H chemical shifts434

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CRO-K56-[DGEVK]1

Entities:

Entity 1, CRO-K56-[DGEVK] 71 residues - Formula weight is not available

1   METGLUGLNARGILETHRLEULYSASPTYR
2   ALAMETARGPHEGLYGLNTHRLYSTHRALA
3   LYSASPLEUGLYVALTYRGLNSERALAILE
4   ASNLYSALAILEHISALAGLYARGLYSILE
5   PHELEUTHRILEASNALAASPGLYSERVAL
6   TYRALAGLUGLUVALLYSASPGLYGLUVAL
7   LYSPROPHEPROSERASNLYSLYSTHRTHR
8   ALA

Samples:

sample_one: CRO Repressor Insertion Mutant K56-[DGEVK] mM; H2O 90%; D2O 10%

sample_conditions_one: ionic strength: 0.02 M; pH: 4.6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
NOESYsample_onenot availablenot available
COSYsample_onenot availablenot available
TOCSYsample_onenot availablenot available
HSQC-NOESYsample_onenot availablenot available
HSQC-TOCSYsample_onenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Varian UNITY-plus 500 MHz
  • Varian UNITY-plus 600 MHz

Related Database Links:

PDB
GB EEW86110