BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4208

Title: The Y64A Mutant of Cytochrome c553 from Desulfovibrio vulgaris Hildenborough   PubMed: 7844834

Authors: SEBBAN-KREUZER, C.; BLACKLEDGE, M.; DOLLA, A.; MARION, D.; GUERLESQUIN, F.

Citation: Blackledge, M.; Medvedeva, S.; Poncin, M.; Guerlesquin, F.; Bruschi, M.; Marion, D.. "Structure and Dynamics of Ferrocytochrome c553 from Desulfovibrio vulgaris Studied by Spectroscopy and Restrained Molecular Dynamics NMR"  J. Mol. Biol. 245, 661-681 (1995).

Assembly members:
CytC(Y64A), polymer, 79 residues, Formula weight is not available
HEM, non-polymer, 616.487 Da.

Natural source:   Common Name: Desulfovibrio desulfuricans   Taxonomy ID: 876   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Desulfovibrio desulfuricans

Experimental source:   Production method: recombinant technology   Host organism: Desulfovibrio desulfuricans

Entity Sequences (FASTA):
CytC(Y64A): ADGAALYKSCIGCHGADGSK AAMGSAKPVKGQGAEELYKK MKGYADGSYGGERKAMMTNA VKKASDEELKALADYMSKL

Data sets:
Data typeCount
1H chemical shifts473

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CytC(Y64A)1
2heme2

Entities:

Entity 1, CytC(Y64A) 79 residues - Formula weight is not available

1   ALAASPGLYALAALALEUTYRLYSSERCYS
2   ILEGLYCYSHISGLYALAASPGLYSERLYS
3   ALAALAMETGLYSERALALYSPROVALLYS
4   GLYGLNGLYALAGLUGLULEUTYRLYSLYS
5   METLYSGLYTYRALAASPGLYSERTYRGLY
6   GLYGLUARGLYSALAMETMETTHRASNALA
7   VALLYSLYSALASERASPGLUGLULEULYS
8   ALALEUALAASPTYRMETSERLYSLEU

Entity 2, heme - C34 H32 Fe N4 O4 - 616.487 Da.

1   HEM

Samples:

sample_one: CytC(Y64A) mM

sample_conditions_one: ionic strength: 10 mM; pH: 5.9; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
NOESYsample_onenot availablesample_conditions_one
HOHAHAsample_onenot availablesample_conditions_one

Software:

No software information available

NMR spectrometers:

  • Bruker AMX 600 MHz

Related Database Links:

PDB
GB AAA16453 AAA23356 AAS96294 ABM28361 ADP86641
REF WP_010939104 YP_011035
SP P04032