BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4214

Title: N-terminal Domain of Tissue Inhibitor of Metalloproteinase-2 (N-TIMP-2)   PubMed: 9705310

Authors: Muskett, F.; Frenkiel, T.; Feeney, J.; Freedman, R.; Carr, M.; Williamson, R.

Citation: Muskett, F.; Frenkiel, T.; Feeney, J.; Freedman, R.; Carr, M.; Williamson, R.. "High resolution structure of the N-terminal domain of tissue inhibitor of metalloproteinases-2 and characterization of its interaction site with matrix metalloproteinase-3"  J. Biol. Chem. 273, 21736-21743 (1998).

Assembly members:
tissue inhibitor of metalloproteinases-2 (TIMP-2), polymer, 127 residues, 14084 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
tissue inhibitor of metalloproteinases-2 (TIMP-2): CSCSPVHPQQAFCNADVVIR TKAVSEKEVDSGNDIYGNPI KRIQYEIKQIKMFKGPEKDI EFIYTAPSSAVCGVSLDVGG KKEYLIAGKAEGDGKMHITL CDFIVPWDTLSTTQKKSLNH RYQMGCE

Data sets:
Data typeCount
13C chemical shifts372
15N chemical shifts128
1H chemical shifts828
coupling constants164

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1N-TIMP-2 monomer1

Entities:

Entity 1, N-TIMP-2 monomer 127 residues - 14084 Da.

1   CYSSERCYSSERPROVALHISPROGLNGLN
2   ALAPHECYSASNALAASPVALVALILEARG
3   THRLYSALAVALSERGLULYSGLUVALASP
4   SERGLYASNASPILETYRGLYASNPROILE
5   LYSARGILEGLNTYRGLUILELYSGLNILE
6   LYSMETPHELYSGLYPROGLULYSASPILE
7   GLUPHEILETYRTHRALAPROSERSERALA
8   VALCYSGLYVALSERLEUASPVALGLYGLY
9   LYSLYSGLUTYRLEUILEALAGLYLYSALA
10   GLUGLYASPGLYLYSMETHISILETHRLEU
11   CYSASPPHEILEVALPROTRPASPTHRLEU
12   SERTHRTHRGLNLYSLYSSERLEUASNHIS
13   ARGTYRGLNMETGLYCYSGLU

Samples:

sample_1: tissue inhibitor of metalloproteinases-2 (TIMP-2), [U-96% 13C; U-98% 15N], 1.4 mM; Sodium Phosphate 25 mM; potassium Chloride 100 mM

sample_cond_1: ionic strength: 125 mM; pH*: 6.7; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1not availablesample_cond_1
2D TOCSYsample_1not availablesample_cond_1
2D COSYsample_1not availablesample_cond_1
3D NOESY-TOCSYsample_1not availablesample_cond_1
2D HSQCsample_1not availablesample_cond_1
3D 15N NOESY HSQCsample_1not availablesample_cond_1
3D 15N HNHAsample_1not availablesample_cond_1
3D 15N HNHBsample_1not availablesample_cond_1
3D HCCH TOCSYsample_1not availablesample_cond_1
3D CBCA(CO)NHsample_1not availablesample_cond_1
3D CBCAHNsample_1not availablesample_cond_1
3D 13C HMQC NOESYsample_1not availablesample_cond_1

Software:

NMRPipe v7/9/97 - NMR data processing

XEASY v1.13.11 - Spectral analysis

DYANA v1.4 - Structure Calculation

NMR spectrometers:

  • Varian UnityPlus 600 MHz
  • Varian Inova 600 MHz

Related Database Links:

PDB
DBJ BAG57573
EMBL CAA38400 CAA44491 CAA53528 CAC35060
GB AAA40445 AAA40446 AAA59581 AAA61186 AAA84581
PIR I53415
REF NP_001166495 NP_003246 NP_035724 NP_068824 XP_001146829
SP P16035 P25785 P30121 Q60453 Q9WUC6