BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4217

Title: The 3D structure of the streptomyces metalloproteinase inhibitor, SMPI, isolated from streptomyces nigrescens TK-23

Authors: Tate, S.; Ohno, A.; Seeram, S.; Hiraga, K.; Oda, K.; Kainosho, M.

Citation: Ohno, A.; Tate, S.; Seeram, S.; Hiraga, K.; Oda, K.; Swindells, M.; Kainosho, M.. "NMR structure of the streptomyces metalloproteinase inhibitor, SMPI, isolated from Streptomyces nigrescens TK-23: another example of an ancestral beta gamma-crystallin precursor structure."  J. Mol. Biol. 282, 421-433 (1998).

Assembly members:
metalloproteinase inhibitor, polymer, 102 residues, Formula weight is not available

Natural source:   Common Name: Streptomyces nigrescens   Taxonomy ID: 1920   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptomyces nigrescens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
metalloproteinase inhibitor: APSCPAGSLCTYSGTGLSGA RTVIPASDMEKAGTDGVKLP ASARSFANGTHFTLRYGPAR KVTCVRFPCYQYATVGKVAP GAQLRSLPSPGATVTVGQDL GD

Data sets:
Data typeCount
13C chemical shifts191
15N chemical shifts97
1H chemical shifts623

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SMPI1

Entities:

Entity 1, SMPI 102 residues - Formula weight is not available

1   ALAPROSERCYSPROALAGLYSERLEUCYS
2   THRTYRSERGLYTHRGLYLEUSERGLYALA
3   ARGTHRVALILEPROALASERASPMETGLU
4   LYSALAGLYTHRASPGLYVALLYSLEUPRO
5   ALASERALAARGSERPHEALAASNGLYTHR
6   HISPHETHRLEUARGTYRGLYPROALAARG
7   LYSVALTHRCYSVALARGPHEPROCYSTYR
8   GLNTYRALATHRVALGLYLYSVALALAPRO
9   GLYALAGLNLEUARGSERLEUPROSERPRO
10   GLYALATHRVALTHRVALGLYGLNASPLEU
11   GLYASP

Samples:

sample_1: metalloproteinase inhibitor mM

sample_cond_1: pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
Heteronuclear multi-dimensional experimentsample_1not availablesample_cond_1

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

PDB
DBJ BAA00574
PRF 1102216A
SP P01077