BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4222

Title: High-Resolution Solution Structure of a Sweet Protein Single-Chain Monellin (SCM) determined by Nuclear Magnetic Resonance Spectroscopy and Dynamical Simulated Annealing Calculations   PubMed: 10029527

Authors: Lee, Seok-Yong; Lee, Jung-Hoon; Chang, Ho-Jin; Cho, JM; Jung, Jin-Won; Lee, Weontae

Citation: Lee, Seok-Yong; Lee, Jung-Hoon; Chang, Ho-Jin; Cho, JM; Jung, Jin-Won; Lee, Weontae. "Solution structure of a sweet protein single-chain monellin determined by nuclear magnetic resonance and dynamical simulated annealing calculations"  Biochemistry 38, 2340-2346 (1999).

Assembly members:
monellin, polymer, 94 residues, Formula weight is not available

Natural source:   Common Name: Serendipity Berry   Taxonomy ID: 3457   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Dioscoreophyllum cumminsii

Experimental source:   Production method: recombinant technology   Host organism: Saccharomyces cerevisiae

Entity Sequences (FASTA):
monellin: GEWEIIDIGPFTQNLGKFAV DEENKIGQYGRLTFNKVIRP CMKKTIYENEREIKGYEYQL YVYASDKLFRADISEDYKTR GRKLLRFNGPVPPP

Data sets:
Data typeCount
15N chemical shifts86
1H chemical shifts513

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1monellin1

Entities:

Entity 1, monellin 94 residues - Formula weight is not available

1   GLYGLUTRPGLUILEILEASPILEGLYPRO
2   PHETHRGLNASNLEUGLYLYSPHEALAVAL
3   ASPGLUGLUASNLYSILEGLYGLNTYRGLY
4   ARGLEUTHRPHEASNLYSVALILEARGPRO
5   CYSMETLYSLYSTHRILETYRGLUASNGLU
6   ARGGLUILELYSGLYTYRGLUTYRGLNLEU
7   TYRVALTYRALASERASPLYSLEUPHEARG
8   ALAASPILESERGLUASPTYRLYSTHRARG
9   GLYARGLYSLEULEUARGPHEASNGLYPRO
10   VALPROPROPRO

Samples:

sample_1: monellin mM

sample_cond_1: pH: 7.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
TOCSYsample_1not availablesample_cond_1
NOESYsample_1not availablesample_cond_1
DQF-COSYsample_1not availablesample_cond_1
1H-15N HSQCsample_1not availablesample_cond_1
1H-15N HMQC-Jsample_1not availablesample_cond_1
1H-15N NOESY-HSQCsample_1not availablesample_cond_1
1H-15N HOHAHA-HMQCsample_1not availablesample_cond_1

Software:

No software information available

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

BMRB 4638
PDB
GB AAG21344 AFF58925
PRF 761801B
SP P02882