BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4223

Title: Solution Structure of a TBP-TAFII230 Complex: Protein Mimicry of the Minor Groove Surface of the TATA Box Unwound by TBP

Authors: Liu, D.; Ishima, R.; Tong, K.; Baby, S.; Kokubo, T.; Muhandiram, D.; Kay, L.; Nakatani, Y.; Ikura, M.

Citation: Liu, Dingjiang; Ishima, R.; Tong, K.; Bagby, S.; Kokubo, T.; Muhandiram, D.; Kay, L.; Nakatani, Y.; Ikura, M.. "Solution Structure of a TBP-TAFII230 Complex: Protein Mimicry of the Minor Groove Surface of the TATA Box Unwound by TB"  Cell 94, 573-583 (1998).

Assembly members:
TATA box binding protein associated factor, polymer, 67 residues, Formula weight is not available

Natural source:   Common Name: Drosophila   Taxonomy ID: 7215   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila not available

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
TATA box binding protein associated factor: EGSIGNGLDLTGILFGNIDS EGRLLQDDDGEGRGGTGFDA ELRENIGSLSKLGLDSMLLE VIDLKEA

Data sets:
Data typeCount
13C chemical shifts239
15N chemical shifts69
1H chemical shifts400

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TAFII2301

Entities:

Entity 1, TAFII230 67 residues - Formula weight is not available

1   GLUGLYSERILEGLYASNGLYLEUASPLEU
2   THRGLYILELEUPHEGLYASNILEASPSER
3   GLUGLYARGLEULEUGLNASPASPASPGLY
4   GLUGLYARGGLYGLYTHRGLYPHEASPALA
5   GLULEUARGGLUASNILEGLYSERLEUSER
6   LYSLEUGLYLEUASPSERMETLEULEUGLU
7   VALILEASPLEULYSGLUALA

Samples:

sample_1: TATA box binding protein associated factor, [U-13C; U-15N; U60%-2H], 1.0 mM

sample_2: TATA box binding protein associated factor, [U-13C; U-15N], 1.0 mM

sample_cond_1: ionic strength: 0.75 M; pH: 7.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
not availablenot availablenot availablesample_cond_1

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

PDB
GB AAB26991 AAD19815 AAF54102 AAO47866 AAS65116
REF NP_001163532 NP_001246952 NP_001287201 NP_476956 NP_996159
SP P51123