BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4231

Title: Artificial Fe8S8 ferredoxin: the D13C variant of Bacillus schlegelii Fe7S8 ferredoxin   PubMed: 9874217

Authors: Aono, S.; Bentrop, D.; Bertini, I.; Cosenza, G.; Luchinat, C.

Citation: Aono, S.; Bentrop, D.; Bertini, I.; Cosenza, G.; Luchinat, C.. "Solution structure of the first artficial Fe8S8 ferredoxin: the D13C variant of Bacillus schlegelii Fe7S8 ferredoxin"  Eur. J. Biochem. 258, 502-514 (1998).

Assembly members:
ferredoxin, polymer, 77 residues, Formula weight is not available
FS4, non-polymer, 351.640 Da.

Natural source:   Common Name: Bacillus schlegelii   Taxonomy ID: 1484   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus schlegelii

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
ferredoxin: AYVITEPCIGTKCASCVEVC PVDCIHEGEDQYYIDPDVCI DCGACEAVCPVSAIYHEDFV PEEWKSYIQKNRDFFKK

Data sets:
Data typeCount
1H chemical shifts653

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ferredoxin1
2IRON/SULFUR CLUSTER I2
3IRON/SULFUR CLUSTER II2

Entities:

Entity 1, ferredoxin 77 residues - Formula weight is not available

1   ALATYRVALILETHRGLUPROCYSILEGLY
2   THRLYSCYSALASERCYSVALGLUVALCYS
3   PROVALASPCYSILEHISGLUGLYGLUASP
4   GLNTYRTYRILEASPPROASPVALCYSILE
5   ASPCYSGLYALACYSGLUALAVALCYSPRO
6   VALSERALAILETYRHISGLUASPPHEVAL
7   PROGLUGLUTRPLYSSERTYRILEGLNLYS
8   ASNARGASPPHEPHELYSLYS

Entity 2, IRON/SULFUR CLUSTER I - Fe4 S4 - 351.640 Da.

1   FS4

Samples:

sample_1: ferredoxin mM

sample_cond_1: ionic strength: 20 mM; pH: 6.5; pressure: 1 atm; temperature: 300 K

sample_cond_2: ionic strength: 20 mM; pH: 6.5; pressure: 1 atm; temperature: 282 K

Experiments:

NameSampleSample stateSample conditions
NOESYsample_1not availablenot available
COSYsample_1not availablenot available
TOCSYsample_1not availablenot available
1D-NOEsample_1not availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

BMRB 4229 4268
PDB
DBJ BAA06187
SP Q45560