BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4232

Title: N-Domain of Troponin C from Chicken Skeletal Muscle.

Authors: Tsuda, Sakae; Miura, A.; Gagne, S.; Spyracopoulos, L.; Sykes, Brian

Citation: Tsuda, Sakae; Miura, A.; Gagne, S.; Spyracopoulos, L.; Sykes, Brian. "Low Temperature-Induced Structural Changes in the Apo Regulatory Domain of Skeletal Muscle Troponin C"  Biochemistry 38, 5693-5700 (1999).

Assembly members:
apoTroponin-C, polymer, 90 residues, Formula weight is not available

Natural source:   Common Name: Chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
apoTroponin-C: ASMTDQQAEARAFLSEEMIA EFKAAFDMFDADGGGDISTK ELGTVMRMLGQNPTKEELDA IIEEVDEDGSGTIDFEEFLV MMVRQMKEDA

Data typeCount
13C chemical shifts259
15N chemical shifts184
1H chemical shifts770
coupling constants81

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1apoNTnC1

Entities:

Entity 1, apoNTnC 90 residues - Formula weight is not available

1   ALASERMETTHRASPGLNGLNALAGLUALA
2   ARGALAPHELEUSERGLUGLUMETILEALA
3   GLUPHELYSALAALAPHEASPMETPHEASP
4   ALAASPGLYGLYGLYASPILESERTHRLYS
5   GLULEUGLYTHRVALMETARGMETLEUGLY
6   GLNASNPROTHRLYSGLUGLULEUASPALA
7   ILEILEGLUGLUVALASPGLUASPGLYSER
8   GLYTHRILEASPPHEGLUGLUPHELEUVAL
9   METMETVALARGGLNMETLYSGLUASPALA

Samples:

sample_one: apoTroponin-C, [U-13C; U-15N], 1.5 mM; KCl 50 mM

sample_conditions_one: pH: 6.7; pressure: 1 atm; temperature: 277 K

Experiments:

NameSampleSample stateSample conditions
CBCACONNHsample_onenot availablesample_conditions_one
15N-NOESYsample_onenot availablesample_conditions_one
HNHAsample_onenot availablesample_conditions_one

Software:

No software information available

NMR spectrometers:

  • Varian UNITY 500 MHz
  • Varian UNITY 600 MHz

Related Database Links:

BMRB 16414 4401
PDB
GB EMP28292 KFU86923
REF XP_007067817 XP_009321443 XP_009582608 XP_009693659 XP_009992324