BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4242

Title: Motile Major Sperm Protein (MSP) of Ascaris suum

Authors: Haaf, Andreas; LeClaire, Lawrence; Roberts, Gregory; Kent, Helen; Roberts, Thomas; Stewart, Murray; Neuhaus, David

Citation: Haaf, Andreas; LeClaire, Lawrence; Roberts, Gregory; Kent, Helen; Roberts, Thomas; Stewart, Murray; Neuhaus, David. "Solution Structure of the Motile Major Sperm Protein (MSP) of Ascaris suum - Evidence for Two Manganese Binding Sites and the Possible Role of Divalent Cations in Filament Formation."  J. Mol. Biol. 284, 1611-1623 (1998).

Assembly members:
Major Sperm Protein, polymer, 126 residues, Formula weight is not available

Natural source:   Common Name: Pig Roundworm   Taxonomy ID: 6253   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Ascaris suum

Experimental source:   Production method: recombinant_technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Major Sperm Protein: AQSVPPGDINTQPSQKIVFN APYDDKHTYHIKITNAGGRR IGWAIKTTNMRRLSVDPPCG VLDPKEKVLMAVSCDTFNAA TEDLNNDRITIEWTNTPDGA AKQFRREWFQGDGMVRRKNL PIEYNL

Data sets:
Data typeCount
13C chemical shifts410
15N chemical shifts132
1H chemical shifts825

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MSP subunit A1
2MSP subunit B1

Entities:

Entity 1, MSP subunit A 126 residues - Formula weight is not available

1   ALAGLNSERVALPROPROGLYASPILEASN
2   THRGLNPROSERGLNLYSILEVALPHEASN
3   ALAPROTYRASPASPLYSHISTHRTYRHIS
4   ILELYSILETHRASNALAGLYGLYARGARG
5   ILEGLYTRPALAILELYSTHRTHRASNMET
6   ARGARGLEUSERVALASPPROPROCYSGLY
7   VALLEUASPPROLYSGLULYSVALLEUMET
8   ALAVALSERCYSASPTHRPHEASNALAALA
9   THRGLUASPLEUASNASNASPARGILETHR
10   ILEGLUTRPTHRASNTHRPROASPGLYALA
11   ALALYSGLNPHEARGARGGLUTRPPHEGLN
12   GLYASPGLYMETVALARGARGLYSASNLEU
13   PROILEGLUTYRASNLEU

Samples:

sample_one: Major Sperm Protein2.5 – 3.5 mM; D2O 5%; H2O 95%

sample_two: Major Sperm Protein, [U-98%15N], 2.5 – 3.5 mM; D2O 5%; H2O 95%

sample_three: Major Sperm Protein, [U-98%13C;U-98%15N], 2.5 – 3.5 mM; D2O 5%; H2O 95%

sample_conditions_one: pH: 4.5; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
NOESYnot availablenot availablenot available
TOCSYnot availablenot availablenot available
DQ-correlationnot availablenot availablenot available
DQF-COSYnot availablenot availablenot available
3D 15N HSQC-NOESYnot availablenot availablenot available
3D 13C HSQC-NOESYnot availablenot availablenot available
HCCHnot availablenot availablenot available

Software:

FELIX v2.30 - processing and analysis

NMR spectrometers:

  • Bruker AMX500 500 MHz
  • Bruker DMX600 600 MHz

Related Database Links:

PDB
EMBL CAA63933
GB AAA29375 AAB23264 AAP76176 ERG81453
SP P27439