BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4244

Title: NMR Solution Structure of [d(GCGAAT-3'-3'-alphaT-5'-5'-CGC)2]   PubMed: 9837995

Authors: Aramini, J.; Mujeeb, A.; Germann, M.

Citation: Aramini, J.; Mujeeb, A.; Germann, M.. "NMR Solution Structures of [d(GCGAAT-3'-3'-alphaT-5'-5'-CGC)2] and its Unmodified Control"  Nucleic Acids Res. 26, 5644-5654 (1998).

Assembly members:
alphaT decamer DNA, polymer, 10 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
alphaT decamer DNA: GCGAATXCGC

Data sets:
Data typeCount
1H chemical shifts94
31P chemical shifts9

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1alphaT strand-A1
2alphaT strand-B1

Entities:

Entity 1, alphaT strand-A 10 residues - Formula weight is not available

1   DGDCDGDADADTATDDCDGDC

Samples:

sample_one: alphaT decamer DNA 6.8 mM; NaCl 50 mM; Na phosphate 10 mM; EDTA 0.1 mM; D2O 100%

sample_two: alphaT decamer DNA 6.8 mM; NaCl 50 mM; Na phosphate 10 mM; EDTA 0.1 mM; D2O 10%; H2O 90%

sample_conditions_one: pH*: 6.5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
NOESYsample_onenot availablenot available
DQF-COSYsample_onenot availablenot available

Software:

XWINNMR v1.1 - data acquisition and processing

SPARKY v3.33 - assignment and cross-peak integration

NMR spectrometers:

  • Bruker AMX 600 MHz

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