BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4246

Title: 3D Structure of the M8L Mutant of Squash Trypsin Inhibitor CMTI-I, NMR, 6 Structures

Authors: Zhukov, Igor; Bolewska, Krystina; Bierzynski, Andrzej

Citation: Zhukov, Igor; Bolewska, Krystina; Bierzynski, Andrzej. "Conservative Mutation Met8-->Leu Affects the Folding Process and Structural Stability of Squash Trypsin Inhibitor CMTI-I"  Protein Sci. 9, 273-279 (2000).

Assembly members:
CMTI-I(M8L), polymer, 29 residues, Formula weight is not available

Natural source:   Common Name: squash   Taxonomy ID: 3661   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Cucurbita maxima

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
CMTI-I(M8L): RVCPRILLECKKDSDCLAEC VCLEHGYCG

Data sets:
Data typeCount
1H chemical shifts198

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1CMTI-I(M8L)1

Entities:

Entity 1, CMTI-I(M8L) 29 residues - Formula weight is not available

1   ARGVALCYSPROARGILELEULEUGLUCYS
2   LYSLYSASPSERASPCYSLEUALAGLUCYS
3   VALCYSLEUGLUHISGLYTYRCYSGLY

Samples:

sample_one: CMTI-I(M8L) mM; NaCl 50 mM

sample_conditions_one: pH: 5.6; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
NOESYsample_onenot availablesample_conditions_one
TOCSYsample_onenot availablesample_conditions_one
DQF-COSYsample_onenot availablesample_conditions_one

Software:

XEASY v3.21 - assignment and integration of two-dimensional NMR spectra

NMR spectrometers:

  • Varian UNITYplus 500 MHz

Related Database Links:

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