BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4249

Title: Solution Structure of the DNA- and RPA-binding Domain of the Human Repair Factor XPA

Authors: Ikegami, Takahisa; Kuraoka, Isao; Saijo, Masafumi; Kodo, Naohiko; Kyogoku, Yoshimasa; Morikawa, Kosuke; Tanaka, Kiyoji; Shirakawa, Masahiro

Citation: Ikegami, Takahisa; Kuraoka, Isao; Saijo, Masafumi; Kodo, Naohiko; Kyogoku, Yoshimasa; Morikawa, Kosuke; Tanaka, Kiyoji; Shirakawa, Masahiro. "Solution Structure of the DNA- and RPA-binding Domain of the Human Repair Factor XPA"  Nat. Struct. Biol. 5, 701-706 (1998).

Assembly members:
XPA, polymer, 122 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
XPA: MEFDYVICEECGKEFMDSYL MNHFDLPTCDNCRDADDKHK LITKTEAKQEYLLKDCDLEK REPPLKFIVKKNPHHSQWGD MKLYLKLQIVKRSLEVWGSQ EALEEAKEVRQENREKMKQK KF

Data sets:
Data typeCount
13C chemical shifts500
15N chemical shifts120
1H chemical shifts724
coupling constants89

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1XPA1
2Zn2

Entities:

Entity 1, XPA 122 residues - Formula weight is not available

1   METGLUPHEASPTYRVALILECYSGLUGLU
2   CYSGLYLYSGLUPHEMETASPSERTYRLEU
3   METASNHISPHEASPLEUPROTHRCYSASP
4   ASNCYSARGASPALAASPASPLYSHISLYS
5   LEUILETHRLYSTHRGLUALALYSGLNGLU
6   TYRLEULEULYSASPCYSASPLEUGLULYS
7   ARGGLUPROPROLEULYSPHEILEVALLYS
8   LYSASNPROHISHISSERGLNTRPGLYASP
9   METLYSLEUTYRLEULYSLEUGLNILEVAL
10   LYSARGSERLEUGLUVALTRPGLYSERGLN
11   GLUALALEUGLUGLUALALYSGLUVALARG
12   GLNGLUASNARGGLULYSMETLYSGLNLYS
13   LYSPHE

Entity 2, Zn - Zn - 65.409 Da.

1   ZN

Samples:

sample_one: Human NER factor XPA, [U-13C; U-15N], 1.2 mM; zinc 0.02 mM; KCl 150 mM; DTT 10 mM; Deuterated Tris-HCl 50 mM

sample_conditions_one: pH: 7.3; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_onenot availablesample_conditions_one

Software:

NMRPIPE v1.6 - Data processing

PIPP v4.0.4 - Peak picker

NMR spectrometers:

  • Bruker DMX500 500 MHz
  • Bruker DRX500 500 MHz
  • Bruker DRX800 800 MHz

Related Database Links:

PDB
DBJ BAA03403 BAJ20553
GB AAA92883 AAH14965 AAM18969 AAV38325 AAX29766
PRF 1701435A
REF NP_000371 XP_001114093 XP_001156167 XP_002743160 XP_002820062
SP P23025