BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4254

Title: 1H, 15N and 13C Assignments of the DNA Binding Domain of Transcription Factor Mbp1 from Sassharomyces cerevisiae in Both Its Free and DNA Bound Forms and 1H Assignments of the Free DNA

Authors: McIntosh, Pauline; Taylor, Ian; Smerdon, Steven; Frenkiel, Thomas; Lane, Andrew

Citation: McIntosh, Pauline; Taylor, Ian; Smerdon, Steven; Frenkiel, Thomas; Lane, Andrew. "1H, 15N and 13C Assignments of the DNA Binding Domain of Transcription Factor Mbp1 from Saccharomyces cerevisiae in Both Its Free and DNA Bound Forms and 1H Assignments of the Fee DNA"  J. Biomol. NMR 13, 397-398 (1999).

Assembly members:
MLU1-BOX binding protein, polymer, 135 residues, 15436.7 Da.

Natural source:   Common Name: Baker   Taxonomy ID: 4932   Superkingdom: not available   Kingdom: not available   Genus/species: Eukaryota Fungi

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
MLU1-BOX binding protein: SNQIYSARYSGVDVYEFIHS TGSIMKRKKDDWVNATHILK AANFAKAKRTRILEKEVLKE THEKVQGGFGKYQGTWVPLN IAKQLAEKFSVYDQLKPLFD FTQTDGSASPPPAPKHHHAS KVDKLAAALEHHHHH

Data sets:
Data typeCount
13C chemical shifts242
15N chemical shifts126
1H chemical shifts488

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Mbp11

Entities:

Entity 1, Mbp1 135 residues - 15436.7 Da.

1   SERASNGLNILETYRSERALAARGTYRSER
2   GLYVALASPVALTYRGLUPHEILEHISSER
3   THRGLYSERILEMETLYSARGLYSLYSASP
4   ASPTRPVALASNALATHRHISILELEULYS
5   ALAALAASNPHEALALYSALALYSARGTHR
6   ARGILELEUGLULYSGLUVALLEULYSGLU
7   THRHISGLULYSVALGLNGLYGLYPHEGLY
8   LYSTYRGLNGLYTHRTRPVALPROLEUASN
9   ILEALALYSGLNLEUALAGLULYSPHESER
10   VALTYRASPGLNLEULYSPROLEUPHEASP
11   PHETHRGLNTHRASPGLYSERALASERPRO
12   PROPROALAPROLYSHISHISHISALASER
13   LYSVALASPLYSLEUALAALAALALEUGLU
14   HISHISHISHISHIS

Samples:

sample_one: MLU1-BOX binding protein, [U-15N], 0.8 – 1.3 mM

sample_two: MLU1-BOX binding protein, [U-15N;U-13C], 0.8 – 1.3 mM

sample_three: MLU1-BOX binding protein0.8 – 1.3 mM

sample_conditions_one: pH: 7.6; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
not availablenot availablenot availablesample_conditions_one

Software:

No software information available

NMR spectrometers:

    Related Database Links:

    BMRB 4256
    PDB
    DBJ GAA22190
    EMBL CAA52271 CAA98618 CAY78452
    GB AAS64332 AAS64333 AAS64335 AAS64336 AAS64338
    REF NP_010227
    SP P39678
    TPG DAA11800