BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4255

Title: Human Translation Initiation Factor eIF1

Authors: Fletcher, C.; Pestova, T.; Hellen, C.; Wagner, G.

Citation: Fletcher, C.; Pestova, T.; Hellen, C.; Wagner, G.. "Structure and interactions of the translation initiation factor eIF1"  EMBO J. 4, 2631-2637 (1999).

Assembly members:
eIF, polymer, 126 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
eIF: MRGSHHHHHHTDPMSAIQNL HSFDPFADASKGDDLLPAGT EDYIHIRIQQRNGRKTLTTV QGIADDYDKKKLVKAFKKKF ACNGTVIEHPEYGEVIQLQG DQRKNICQFLVEIGLAKDDQ LKVHGF

Data sets:
Data typeCount
13C chemical shifts452
15N chemical shifts128
1H chemical shifts730

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1eIF11

Entities:

Entity 1, eIF1 126 residues - Formula weight is not available

1   METARGGLYSERHISHISHISHISHISHIS
2   THRASPPROMETSERALAILEGLNASNLEU
3   HISSERPHEASPPROPHEALAASPALASER
4   LYSGLYASPASPLEULEUPROALAGLYTHR
5   GLUASPTYRILEHISILEARGILEGLNGLN
6   ARGASNGLYARGLYSTHRLEUTHRTHRVAL
7   GLNGLYILEALAASPASPTYRASPLYSLYS
8   LYSLEUVALLYSALAPHELYSLYSLYSPHE
9   ALACYSASNGLYTHRVALILEGLUHISPRO
10   GLUTYRGLYGLUVALILEGLNLEUGLNGLY
11   ASPGLNARGLYSASNILECYSGLNPHELEU
12   VALGLUILEGLYLEUALALYSASPASPGLN
13   LEULYSVALHISGLYPHE

Samples:

sample_one: eIF, [U-100% 15N], 1 – 2 mM; Sodium Phosphate 50 mM; dtt 1 mM; EDTA 1 mM; Azide 0.1 mM; H2O 90%; D2O 10%

sample_two: eIF, [U-100% 15N; U-100% 13C], 1 – 2 mM; Sodium Phosphate 50 mM; dtt 1 mM; EDTA 1 mM; Azide 0.1 mM; H2O 90%; D2O 10%

sample_conditions_one: ionic strength: 400 mM; pH: 6.0; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Varian UNITYplus 400 MHz
  • Varian INOVA 750 MHz

Related Database Links:

PDB
DBJ BAE28300 BAE29876 BAE30086 BAE30515 BAE31024
EMBL CAA90519 CAD66615 CAG33332 CAH89503
GB AAA60602 AAD19900 AAD52028 AAH03463 AAH05118
REF NP_001014884 NP_001099307 NP_001124650 NP_001161207 NP_001185535
SP P41567 P48024 P51971 Q5E938 Q5RFF4
TPG DAA18448 DAA31349